American Journal of Plant Sciences, 2020, 11, 2080-2102
https://www.scirp.org/journal/ajps
ISSN Online: 2158-2750
ISSN Print: 2158-2742
DOI: 10.4236/ajps.2020.1112147 Dec. 24, 2020 2080 American Journal of Plant Sciences
Screening of Rice Accessions for Tolerance to
Drought and Salt Stress Using Morphological
and Physiological Parameters
Chukwudi Umego
1
, Valentine Otang Ntui
1,2
, Effiom Eyo Ita
1
, Charles Opara
1
, Edak Aniedi Uyoh
1*
1
Department of Genetics and Biotechnology, University of Calabar, Calabar, Nigeria
2
International Institute for Tropical Agriculture, Nairobi, Kenya
Abstract
Drought and salinity are the most widespread soil problems, posing a big
threat to food security in rice growing regions. The present study evaluated
the performance of eleven rice genotypes using morphological and physio-
logical parameters, under induced drought and salinity conditions. The
seedlings were raised in 5 kg of homogenous soil in plastic bags in the green-
house. For the drought experiment, each bag was watered with 200 ml of wa-
ter twice daily until plants reached the five-leaf stage when watering was sus-
pended for 2 weeks for the drought stressed plants but not suspended for the
control plants. The experiment was a 2 × 11 factorial and the set up was ar-
ranged using the completely randomized design with three replications. Data
were taken on Plant height, Number of tillers, leaf length, Number of green
leaves, Number of dead leaves, Leaf rolling score (LRS) and Rate of water loss.
The salinity experiment was set up in a similar manner except that the plants
were irrigated twice a day for 2 weeks with 200 ml of treatment solution con-
taining either 0 mM NaCl or 75 mM and data were collected on plant height,
number of tillers, shoot fresh weight, shoot dry weight, Na
+
and K
+
concen-
trations, relative water content and chlorophyll content. Data from both ex-
periments were subjected to Analysis of variance test using the GenStat soft-
ware 10
th
edition and the means separated using least significant difference
test. Individual stress response index (ISRI) was calculated for each parameter
and the means used in grouping the varieties. Of the genotypes evaluated,
four (FARO 44, NERICA 2, NERICA 8 and NERICA 5) were identified as to-
lerant, two (NERICA 4 and FARO 57) as moderately tolerant, while the rest
were found to be sensitive to drought. Equally, two varieties (FARO 44 and
RAM 137) stood out in the salinity screening as tolerant varieties, five were
moderately tolerant while four (FARO 64, FARO 52, NERICA 2 and FARO
How to cite this paper: Umego, C., Ntui,
V.O., Ita, E.E., Opara, C. and Uyoh, E.A.
(2020) Screening of Rice Accessions for
Tolerance to Drought and Salt Stress Using
Morphological and Physiological Parame-
ters. American Journal of Plant Sciences,
11, 2080-2102.
https://doi.org/10.4236/ajps.2020.1112147
Received: November 7, 2020
Accepted: December 21, 2020
Published: December 24, 2020
Copyright © 2020 by author(s) and
Scientific Research Publishing Inc.
This work is licensed under the Creative
Commons Attribution International
License (CC BY 4.0).
http://creativecommons.org/licenses/by/4.0/
Open Access