1 The Role of Host Calcium Signaling in Toxoplasma gondii egress Stephen A. Vella 1,2 , Christina A. Moore 1,3 , Zhu-Hong Li 1 , Miryam A. Hortua Triana 1 , Evgeniy Potapenko 1 and Silvia N J Moreno 1,3, * From the 1 Center for Tropical and Emerging Global Diseases, 2 Department of Microbiology and 3 Department of Cellular Biology, University of Georgia, Athens, GA, 30602 Running title: Host calcium and Toxoplasma egress * Corresponding author: smoreno@uga.edu Keywords: Toxoplasma gondii, calcium signaling, genetically-encoded calcium indicators, GCaMP6f, RGECO, LAR-GECO, jGCaMP7f, Toxoplasma egress . CC-BY 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted March 6, 2020. ; https://doi.org/10.1101/2020.03.06.980508 doi: bioRxiv preprint