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Volume 1 - Issue 1
Immune Response Associated to Infection
It is known that the consequences of an immune response
against a microorganism are often closely correlated with the
interleukins that are produced by cells of innate or adaptive
immunity. In adaptive immunity, the production of interleukins by
TCD4+ ou T-helper cellsis arranged according to the differentiation
of these cells. They can differentiate into distinct subtypes
characterized as Th1, Th2 e Th17, among others, each of which
has specialized tasks considering the type of interleukin that
is secreted [1]. The TCD4+cell differs in a particular subtype
according to the microorganism involved in the infectious disease.
Microorganisms, when captured by cells of innate immunity,
induce the production of certain interleukins that will direct the
polarization of the TCD4+cells. These interleukins induce the
production of transcription factors that will determine specific
signaling pathways, which are responsible for the production of
interleukins for each of these T cell patterns [2,3].
Th1 is an important standard in the immune response to
viruses, bacteria and parasites through the production of IL-2
and IFN-γ cytokines and Th2 acts in the response especially
against extracellular parasites as a function of the production of
IL-4, IL-5 and IL-6 [4,5]. The first signs of independent Th1/Th2
polarization appeared in the mid-1990s, with the discovery of
IL17.The TCD4+lymphocytes producing IL-17 were termed Th17.
These cells play a key role in the control of the intestinal microbiota
as well in the defense against bacteria and fungi. In vivo and in
vitro studies have demonstrated that IL-17 can initiate or amplify
various inflammatory pathways [6]. IL-17 is of particular interest in
the pathogenesis of periodontal disease due to its role both in the
inflammatory process associated with disease and in the protective
function against the microorganism P. gingivalis. The mechanisms
associated with IL-17 in this context include the ability not only
to orchestrate recruitment of neutrophils to infection sites but
also to stimulate the production of antimicrobial peptides such
as defensins and cathelicidins [7]. The possibility of detecting
interleukins at the site of infection is important in determining the
type of immune response and thereby tracing forms of treatment
and/or monitoring their effect. The detection of interleukins (IL-
Renata Kelly Mendes
1
, Giovanna R Degasperi
2
, Frank Herman Behrens
3
, Larissa Marcelino
4
, Ahron Sicard
2
, Karina
Villalpando
5
, Sérgio L Pinheiro
4,5
and Augusto Etchegaray
1,4
*
1
Faculty of Chemistry, Pontifical Catholic University of Campinas, Brazil
2
Immunology League, Faculty of Medicine, Pontifical Catholic University of Campinas, Brazil
3
Faculty of Electric Engineering, Pontifical Catholic University of Campinas, Brazil
4
Post-Graduation in Health Sciences, Pontifical Catholic University of Campinas, Brazil
5
Dental School, Pontifical Catholic University of Campinas, Brazil
*Corresponding author: Augusto Etchegaray, Faculty of Chemistry, Post-Graduation in Health Sciences, Pontifical Catholic University of Campinas,
Campinas, Brazil
Submission: December 21, 2017; Published: January 12, 2018
Applications and Perspectives of Biosensors for
Diagnostics in Infectious Diseases
Cohesive J Microbiol infect Dis
Copyright © All rights are reserved by Augusto Etchegaray.
CRIM SONpublishers
http://www.crimsonpublishers.com
Abstract
Infectious diseases are considered a major cause of morbidity and/or mortality worldwide, despite the development of preventive and control
strategies. Infectious diseases result from the invasion of body tissues by disease-causing microorganisms. The occurrence and development of
infectious diseases are closely associated with the functional state of the immune system. Interleukins play significant roles in modulating the immune
response of the host during the course of an infectious disease, contributing to the maintenance of the homeostasis of the immune system. The use of
biosensors for the detection of interleukins is a new and important strategy for the diagnosis of the infection and the immunological response profile
that occurs for specific diseases, thus helping in the effective diagnosis, and allowing physicians to design treatment in a faster way, which contributes
to better prognosis. For example, in the oral environment there are two major infectious diseases: caries and periodontitis. The microbes involved are
respectively, Streptococcus mutans and Porphyromonasgingivalis. The presence of specific interleukins in saliva or crevicular fluid will indicate that there
is potentially an infectious disease, which could be connected with either caries or periodontal disease. For instance, an indicative for caries could be
the presence of IL-6 in saliva, while periodontal disease would be confirmed by the presence of IL-17 in crevicular fluid. In addition, both diseases could
be confirmed by the detection of higher levels of each of one of the pathogens. Considering the development of biosensor design and construction, the
answer to all these question could be obtained in a single analysis.
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