ORIGINAL ARTICLE Identification and antifungal susceptibility of fungi isolated from dermatomycoses L.B. Silva, 1 D.B.C. de Oliveira, 1 B.V. da Silva, 1 R.A. de Souza, 1 P.R. da Silva, 1 K. Ferreira-Paim, 2 L.E. Andrade-Silva, 2 M.L. Silva-Vergara, 2 A.A. Andrade 1, * 1 Laboratory of Microbiology, Institute of Biological and Natural Sciences, Federal University of Tri^ angulo Mineiro, Uberaba, Minas Gerais, Brazil 2 Infectious Diseases Unit, Federal University of Tri^ angulo Mineiro, Uberaba, Minas Gerais, Brazil *Correspondence: A.A. Andrade. E-mail: anderson@icbn.uftm.edu.br Abstract Background Dermatomycoses are superficial fungal infections of the skin, hair and nails that affect more than 20–25% of the people worldwide. These infections can be caused by yeasts, dermatophytes and non-dermatophyte filamentous fungi (NDFF) and are considered a public health problem. Despite this, few studies have investigated the prevalence and antifungal susceptibility of causative agents of dermatomycoses in the developing world. Objectives The aims of this study were to identify and determine the antifungal susceptibility profile of yeast and fila- mentous fungi isolated from dermatomycoses in Uberaba, Minas Gerais, Brazil. Methods Specimens were obtained from patients with clinically diagnosed and laboratory confirmed dermatomycosis between July 2009 and July 2011. Fungal identification was based on classical methods and antifungal susceptibility testing was performed by broth microdilution method. Results Of the 216 fungal isolates, 116 (53.8%) were yeasts, 70 (32.4%) dermatophytes and 30 (13.8%) NDFF. Onychomycosis was the most common clinical condition. Candida parapsilosis (24.1%) and Trichophyton rubrum (17.1%) were the fungi most frequently isolated. Voriconazole, ketoconazole and itraconazole were the most potent anti- fungal agents against yeast, whereas terbinafine, voriconazole and itraconazole had a high in vitro activity against der- matophytes. Overall, the antifungal agents had little or no activity against NDFF and the highest minimum inhibitory concentrations were those against Fusarium spp. Conclusion Yeasts, particularly C. parapsilosis, play an important role as causative agents of dermatomycosis in our region. Our results suggest that the antifungal susceptibility testing coupled with proper identification of the fungi may be useful to assist clinicians in determining the appropriate therapy for dermatomycoses. Received: 30 July 2012; Accepted: 6 March 2013 Conflict of interest None declared. Funding sources This work was supported by grants from Fundac ß~ ao de Amparo  a Pesquisa do Estado de Minas Gerais (FAPEMIG) and Fundac ß~ ao de Ensino e Pesquisa de Uberaba (FUNEPU). LBS and RAS were recipients of fellowships from FAPEMIG, and DBCO from Conselho Nacional de Desenvolvimento Científico e Tecnol  ogico (CNPq). BVS, KFP and LEAS are recipients of fellowships from CAPES (Coordenadoria de Aperfeic ßoamento de Pessoal de Nível Superior). Introduction Dermatomycoses are superficial fungal infections of the skin, hair and nails that affect more than 20–25% of the people world- wide, particularly in tropical and subtropical regions, making them one of the most common dermatological conditions. 1,2 These diseases, although are not life-threatening, are considered a public health problem, as they affect the quality of life of individuals. 2,3 The fungi responsible for dermatomycoses include dermato- phytes, yeasts and non-dermatophytic filamentous fungi (NDFF). A number of epidemiological studies have demon- strated that most cases of superficial fungal infections are caused by dermatophytes. 3–5 However, the prevalence of the dermato- mycoses, as well as the distribution of their aetiological agents can vary considerably according to geographical location, climatic conditions, socioeconomic status, population migration © 2013 The Authors Journal of the European Academy of Dermatology and Venereology © 2013 European Academy of Dermatology and Venereology JEADV 2013 DOI: 10.1111/jdv.12151 JEADV