Clinical Study
Urine β-2-Microglobulin, Osteopontin, and Trefoil Factor
3 May Early Predict Acute Kidney Injury and Outcome after
Cardiac Arrest
Sigrid Beitland ,
1,2
Espen Rostrup Nakstad,
3
Jens Petter Berg,
1,4
Anne-Marie Siebke Trøseid,
4
Berit Sletbakk Brusletto,
4
Cathrine Brunborg,
5
Christofer Lundqvist,
1,6
and Kjetil Sunde
1,2
1
Institute of Clinical Medicine, University of Oslo, P.O.Box 1072 Blindern, 0316 Oslo, Norway
2
Department of Anaesthesiology, Oslo University Hospital, P.O.Box 4950 Nydalen, 0424 Oslo, Norway
3
Norwegian National Unit for CBRNE Medicine, Oslo University Hospital, P.O.Box 4956 Nydalen,
0424 Oslo, Norway
4
Department of Medical Biochemistry, Oslo University Hospital, P.O.Box 4950 Nydalen, 0424 Oslo, Norway
5
Oslo Centre for Biostatistics and Epidemiology, Oslo University Hospital, P.O.Box 1122 Blindern,
0317 Oslo, Norway
6
Health Services Research Unit and Department of Neurology, Akershus University Hospital, P.O.Box 1000,
1478 Lørenskog, Norway
Correspondence should be addressed to Sigrid Beitland; sigrid.beitland@medisin.uio.no
Received 2 November 2018; Accepted 9 April 2019; Published 7 May 2019
Academic Editor: omas J. Esposito
Copyright © 2019 Sigrid Beitland et al. is is an open access article distributed under the Creative Commons Attribution
License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is
properly cited.
Purpose. Acute kidney injury (AKI) is a common complication after out-of-hospital cardiac arrest (OHCA), leading to increased
mortality and challenging prognostication. Our aim was to examine if urine biomarkers could early predict postarrest AKI and
patient outcome. Methods. A prospective observational study of resuscitated, comatose OHCA patients admitted to Oslo
University Hospital in Norway. Urine samples were collected at admission and day three postarrest and analysed for β-2-
microglobulin (β2M), osteopontin, and trefoil factor 3 (TFF3). Outcome variables were AKI within three days according to the
Kidney Disease Improving Global Outcome criteria, in addition to six-month mortality and poor neurological outcome (PNO)
(cerebral performance category 3–5). Results. Among 195 included patients (85% males, mean age 60 years), 88 (45%) developed
AKI, 88 (45%) died, and 96 (49%) had PNO. In univariate analyses, increased urine β2M, osteopontin, and TFF3 levels sampled at
admission and day three were independent risk factors for AKI, mortality, and PNO. Exceptions were that β2M measured at day
three did not predict any of the outcomes, and TFF3 at admission did not predict AKI. In multivariate analyses, combining clinical
parameters and biomarker levels, the area under the receiver operating characteristics curves (95% CI) were 0.729 (0.658–0.800),
0.797 (0.733–0.861), and 0.812 (CI 0.750–0.874) for AKI, mortality, and PNO, respectively. Conclusions. Urine levels of β2M,
osteopontin, and TFF3 at admission and day three were associated with increased risk for AKI, mortality, and PNO in comatose
OHCA patients. is trail is registered with NCT01239420.
1. Introduction
Acute kidney injury (AKI) affects around 37% of initial
cardiac arrest (CA) survivors; the condition is associated
with increased morbidity and mortality [1, 2]. Uniform
definitions of AKI based on serum creatinine and urine
output are useful in clinical practice and research, for in-
stance the Kidney Disease Improving Global Outcomes
(KDIGO) criteria [3]. However, a major shortcoming of
these definitions is that AKI is detected quite late, and early
Hindawi
Critical Care Research and Practice
Volume 2019, Article ID 4384796, 9 pages
https://doi.org/10.1155/2019/4384796