69 INTRODUCTION G enus Amanita Pers. (Amanitaceae; Agaricales) has more than 500 species which are distributed worldwide (Kirk et al., 2008; Menolli et al., 2009; Tulloss, 2009; Wartchow et al., 2009; Justo et al., 2010; Wartchow & Gamboa-Trujillo, 2012; Cho et al., 2015; Hosen et al., 2015; Tang et al., 2015; Wartchow & Cortez, 2016). Whereas, most of the regional species of the genus are still unknown (Bas, 2000; Tulloss, 2005). Ectomycorrhizal symbiosis is the most important character of the genus. Except some species, all are found to be associated with trees belonging to different types of genera and play a vital part in ecosystems (Yang, 1997, 2000; Wolfe, 2012). Till now, 18 Amanita Species are identified in Pakistan (Ahmad et al. 1997; Tulloss et al. 2001). Other novel species of Amanita have also been reported, but due to a lack of critical data necessary for species description their identity remains unknown to the mycological community (Ahmad et al., 1997; Tulloss et al., 2001). Many taxa of the genus have been reported as comestibles (Buyck, 1994; Montoya-Esquivel, 1997; Tulloss & Bhandary, 1992). Family Amanitaceae is characterized by white Re-investigation of Amanita flavipes : I dentification and Characterization Based on rDNA-I TS Sequence, M orphology and Anatomy Muhammad Hanif* and Samar Bashir Department of Botany, GC University, Lahore Corresponding Author’s Email:.dr.mhanif@gcu.edu.pk LGU Journal of LIFE SCIENCES LGU J.Life.Sci ISSN 2519-9404 eISSN 2521-0130 Research Article Vol 2 issue 1 Jan-March 2018 LGU Society of Life Sciences Hanif & Bashir LGU J. Life. Sci. 2018 LGU J. Life Sci 2(1):LGUJLS MS.ID-038 (2018) Re-investigation of Amanita flavipes: identification and characterization ABSTRACT: Swat valley is Himalayan moist temperate forest of Pakistan. It is also called mini Switzer- land of Pakistan. This area has diverse flora. A wide diversity of mushrooms is present over here but number of species explored is very small. In the present study, a species of Amanita was collected during field tour and identified on the basis of morpho-anatomical and molecular markers which confirmed its identification as Amanita flavipes. BLAST analysis of this species has been re-investigated from new locality swat and is being reported first time from this area. Keywords: Ellipsoidal spores, Miandam, Phylogenetic, section Validae, Amanita flavipes.