260 Analgesic Activity of Water Extract of Binahong (anredera cordifolia (ten.) V. Steenis) Leaves on Swiss Webster Mice Dominus MBunga School of Pharmacy, Bandung Institute of Technology Stefany Fernandez Health Polytechnic of Kupang Email: noezz.bunga@gmail.com Abstract Pain is symptom of disease with high prevalence in the world. One model for therapy that may have benefit in reducing pain is using binahong (Anredera cordifolia (Ten.) v. Steenis) leaves, which was traditionally used by community. There was no study that proved analgesic activity of binahong leaves, but many researches were performed scientifically for many diseases. This research aimed to prove peripheral analgesic activity of binahong leaves scientifically and to determine the effective dose that could provide activity. Extraction was conducted by reflux method. Analgesic peripheral activity of water extract of binahong leaves with doses of 50 mg/kg bw, 100 mg/kg bw and 200 mg/kg bw were performed which induced by acetic acid 0.6 % (Siegmund method). This study showed that all doses of water extract of binahong leaves had analgesic activity which was significantly different with control (p<0,05). Water extract of binahong leaves with doses of 100 mg/kg bw and 200 mg/kg bw had the highest analgesic activity which were not significantly different compared to aspirin. Writhing numbers of doses 100 mg/kg bw and 200 mg/kg bw respectively were 29.60 ± 13.45 and 26.60 ± 11.89 compared to aspirin 21.00 ± 23.21 and % protection of doses 100 mg/kg bw and 200 mg/kg bw respectively were 65.90 and 69.35 compared to aspirin 75.81. Keywords: analgesic, anredera cordifolia, binahong, pain *Correspondence: noezz.bunga@gmail.com Present Address: Kupang, East Nusa Tenggara, Indonesia - 85111 ©The Author(s) 2018. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. O R A L