Tetty Noverita Khairan IJAMS Research Study Brine Shrimp Lethality T Vin Tetty Noverita Khairani* 1 , K Fhitriana Pakpahan 1 1 Faculty of Pharmacy and Health 2 Faculty of Public Health, Institu *Corresponding Author: Tetty No Email id: tettynoverita@helvetia.ac ABSTRACT Vinca (Catharanthus roseus) is a pl determine the percentage of mortali against the larvae of Artemia salina L the Brine Shrimp Lethality Test (BS ppm, 60 ppm, 100 ppm, 140 ppm, a treatments, namely, 0 ppm, 200 ppm, animals were used, Artemia salina L percentage of mortality of Artemi Concentration (LC 50 ) value. The res analysis is y = 3.0809 x - 12.065 wit extract is y = 15.10178 x- 30.0269 w flowers are toxic because they have a Keywords: BSLT, Catharanthus r INTRODUCTION Medicinal plants have long been of Indonesia in an effort to cure an increase endurance and restore fitne that Indonesia is a country that has around 30 thousand types of plants than 1000 types have been used for m [1]. These research centers use th methods to analyze biologically a Apart from efficacy and quality, a f considered is the safety of traditiona use of herbal preparations as treatme medicinal plants is the vinca pla roseus). Catharanthus roseus is an im ni et al / Int. J. of Allied Med. Sci. and Clin. Research Vol - 181 SCR |Volume 9 | Issue 2 | Apr - Jun - 2021 www.ijamscr.com Test (BSLT) of Methanol and Ethyl A nca Flower (Catharanthus roseus) Khairani Fitri 1 , Vivi Eulis Diana 1 , Endang M h, Institut Kesehatan Helvetia, Medan, Indonesia ut Kesehatan Helvetia, Medan, Indonesia overita Khairani c.id lant that belongs to the Apocynaceae family. The pu ity data and the LC 50 value of the Vinca flower extr Leach using the Brine Shrimp Lethality Test (BSLT) m SLT). The methanol extract test consisted of six trea and 180 ppm, and the ethyl acetate extract test consis , 220 ppm, 240 ppm, 260 ppm, 280 ppm, 300 ppm. At Leach larvae aged 48 hours. The toxic effect of the ex ia salina Leach larvae using a probit analysis to sults of the study in the form of a linear regression th a value of LC 50 = 34.599 ppm. The linear regression with a value of LC 50 = 208.636 ppm. The conclusion an LC50 value <1000 ppm. roseus, Extract, Toxicity. used by the people nd prevent disease, ess. As it is known s rich biodiversity, in Indonesia, more medicinal purposes he latest scientific active ingredients. factor that must be al medicines in the ent [2,3]. One of the ant (Catharanthus mportant medicinal plant in the Apocynaceae fa is a species endemic to Mad flower colors, namely the pi roseus and the white flower According to Verrananda (2 stated that the results of contained alkaloids, flavono terpenoids. The secondary m in Catharanthus roseus sh activity as an antidote to fr studies have been carried o and benefits, including hyperglycemic and hypoglyc and diabetes effects. Accor conducted by Prasetyo (2016 International Journal of Allied Me and Clinical Research (IJA -9(2) 2021 [181-185] Medical research Acetate Extract of Marianti 2 , Suzan urpose of this study was to ract (Catharanthus roseus) method. The method used is atments, namely 0 ppm, 20 sted of seven concentration t each concentration, 10 test xtract was identified by the o obtain a 50% Lethality n equation from the probit n equation for ethyl acetate n of this study is that Vinca amily. Catharanthus roseus dagascar, has two types of ink flower of Catharanthus r of Catharanthus alba [4]. 2016), in his research, he phytochemical screening oids, phenolics, tannins and metabolites with IC50 values howed natural antioxidant ree radicals [5,6]. Various out to examine its contents vinca which has anti- cemic effects, antibacterial, rding to previous research 6), showing that extracts of ISSN:2347-6567 edical Sciences AMSCR)