OPEN ACCESS Freely available online
J Geogr Nat Disas, Vol.11 Iss.6
6
ABSTRACT
Hexavalent chromium (Cr-VI), widely used in tanning industries, is a potent toxic metal whose accumulation in the
animal body can adversely affect the reproductive organs. District Kasur, Pakistan, is famous for having tanneries
industry where Cr (VI) is directly discharged untreated into the adjoining water nullahs. Resultantly, wildlife
species, reliant on these water sources, are exposed to chromium toxicity, which enters into the animal body through
drinking water and food chain. The current study investigated toxic effects of Cr(VI) on testicular tissue of adult
small Indian mongoose inhabiting the study area from February 2015 to January 2016. Average Cr-concentrations,
in experimental area soil and water, and the blood and tissue samples of the species were found significantly higher
compared to control. Average body and testicular weights of experimental animals were found reduced. Histological
analysis revealed seminiferous tubules disorganized in experimental animals, depleted germ cells and hyperplasia of
the Leydig cells. Sperm counts were found reduced. Serum testosterone and LH levels were found reduced while
FSH levels increased in experimental animals. The study concludes that Cr being discharged from tanneries into
the environment is up taken by small Indian mongoose leading to severe testicular tissue damage and potential
impairment of reproductive function of the species.
Journal of Geography and Natural Disasters
Short Communication
Hexavalent chromium induces testicular dysfunction in small Indian
mongoose (Herpestes javanicus) inhabiting tanneries areas of Kasur
District, Pakistan
Shaista Andleeb and Tariq Mahmood
PMAS-AAUR, Pakistan
Introduction:
Chromium (Cr) is a naturally occurring element that exists in a
variety of oxidation states (−2 to +6). Among the ionic forms of
Cr, hexavalent chromium [Cr(VI)], the most toxic form, can readily
cross cellular membranes via nonspecific anion transporters1.
After entering the cell, Cr(VI) is reduced to produce reactive
intermediates, including Cr(V), Cr(IV), Cr(III) and reactive oxygen
species (ROS). These species can cause DNA strand breaks, base
modifications and lipid peroxidation, thereby disrupting cellular
integrity and inducing toxic, as well as mutagenic effects. Cr(VI)
is used in more than 50 different industries worldwide in a variety
of applications, including pigment and textile production, leather
tanneries, wood processing, chrome plating, metallurgical and
chemical industries, stainless steel factories, welding, cement
manufacturing factories, ceramic, glass and photographic
industries, catalytic converter production for automobiles, heat
resistance and as an anti-rust agent in cooling plants. The increased
use by industries, coupled with improper disposal of Cr(VI) waste,
has resulted in an increase in the levels of Cr(VI) in soil, water
and air, leading to environmental pollution. It is estimated that
approximately half a million workers in the United States and
several million workers worldwide have been exposed to Cr(VI) (via
inhalation and skin contact).
Environmental or occupational exposure to Cr(VI) results in an
increased risk of asthma, nasal septum lesions, skin ulcerations
and cancers of the respiratory system. Cr(VI) is also known
to cause cytotoxic, genotoxic, immunotoxic and carcinogenic
effects in both humans and laboratory animals, as well as allergic
dermatitis and reproductive toxicity. In the welding industry,
workers exposed to Cr(VI) have an increased risk of poor semen
quality and sperm abnormalities that lead to infertility or cause
developmental problems in children. An increase in spermatozoa
with abnormalities and a decrease in sperm count have also been
reported in Cr-treated/exposed mice, rats, rabbits and bonnet
monkeys.
Although Cr(VI) is known to affect male reproductive health,
there is limited scientific data concerning the toxicity and there are
no appropriate in vitro models to clearly understand the possible
cytotoxic effects, including oxidative stress and apoptosis. In the
present study, we investigated the mechanism underlying the toxic
effects of Cr(VI) in male somatic and spermatogonial stem cells
(SSCs). Leydig cells are somatic cells adjacent to the seminiferous
tubules that produce the primary androgen, testosterone, an
important hormone for the maturation of sperm. Sertoli cells are
located in the convoluted seminiferous tubules and are responsible
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