Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved. e68 Abstracts Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved M O N D A Y O R A L OS 08-01 ASSOCIATION OF ACE, FABP2, PPARG2, GST, FTO AND CYP46A1 GENES POLYMORPHISM WITH ESSENTIAL HYPERTENSION AMONG NORTH INDIAN POPULATION Syed Tasleem Raza, Shania Abbas, Farzana Mahdi. Department of Biochemistry, Era’s Lucknow Medical College, India Objective: Hypertension (HTN) is the most common cardiovascular disease and is assuming epidemic proportions in developing countries. Genetic and environ- mental factors play important role in the development of hypertension.The ob- jective of this study was to investigate the possible association of ACE, FABP2, PPARG2, GST, FTO and CYP46A1 genes polymorphism and susceptibility of individuals to HTN. Design and method: This case control study includes 123 essential HTN cases and 102 controls. ACE, GST genes polymorphisms were evaluated by PCR and FABP2, PPARG2, FTO, CYP46A1 genes polymorphisms were evaluated by PCR- RFLP methods. Results: The mean age of cases and controls were 41.32 ± 11.39 years and 40.13 ± 10.28 years. Frequencies of ACE II, ID, DD genotypes in essential HTN cases and controls were 22.76%, 63.42%, 13.82% and 11.77%, 60.78%, 27.45%; respectively. Frequencies of FABP2 AA, AT, TT in cases and controls were 11.38%, 70.74%, 17.88% and 20.59%, 64.71%, 14.70%. Frequencies of PPARG2 CC, CG, GG genotype in cases and control were 27.65%, 65.85%, 6.50% and 21.57%, 37.26%, 41.17%. Frequency of GSTM1 null, GSTT1 null genotypes in cases and controls were 41.47%, 5.69% and 52.95%, 4%; respec- tively. Frequencies of FTO AA, AT, TT genotype in cases and control were 1.63%, 68.29%, 30.08% and NA, 75.49%, 24.51%. Frequencies of CYP46A1 CC, CT, TT genotype in cases and control were 17.08%, 33.33%, 49.59% and 3.93%, 28.43%, 67.64%. Significant differences were obtained in the frequencies of ACE II genotype (p = 0.032), PPARG2 CG, GG genotype (p < 0.001) CYP46A1 CC, TT genotype (p = 0.002, 0.006). Conclusions: Findings of this study suggest that ACE, PPARG2, CYP46A1 genes were significantly associated with essential HTN cases in North Indian populations. OS 08-02 APELIN AND APLN SINGLE NUCLEOTIDE POLYMORPHISMS AND COMBINED HYPERTENSION AND CENTRAL RETINAL ARTERY STENOSIS IN A CHINESE POPULATION Feng Huang, Qiuxia Huang, Fan Lin, Pengli Zhu. Department of Geriatric Medi- cine, Fujian Provincial Hospital, China Objective: Apelin activity plays a role in regulating blood pressure. This study explored the relationship between single nucleotide polymorphisms (SNPs) in the Apelin gene (APLN) with hypertension and hypertension with central retinal ar- tery equivalent (CRAE) stenosis in a coastal Chinese population. Design and method: All subjects answered an epidemiological survey for de- mographic and disease characteristics. Apelin levels were determined and three APLN SNPs, rs56204867, rs3115757, and rs3761581, were evaluated. CRAE was measured using fundus photography. Results: Apelin levels were significantly lower in subjects with hypertension and hypertension with CRAE stenosis (0.23 ± 0.10 ng/ml and 0.21 ± 0.08 ng/ml, re- spectively) compared with control subjects (0.25 ± 0.11 ng/ml; p50.001). Linear regression analysis showed hypertension and hypertension with CRAE stenosis was associated with age, being male, systolic blood pressure, abnormal blood lip- ids, and Apelin levels. Genetic analysis indicated that in both males and females SNP rs3761581 was associated with hypertension and that more males carrying rs56204867 and rs3761581 T-A haplotype had hypertension (61.88%) and hyper- tension with CRAE stenosis (56.82%) than control males (39.33%). Conclusions: In this Chinese population, Apelin and APLN SNP rs3761581 was associated with combined hypertension with CRAE, indicating that the expres- sion of APLN gene products may be involved in vascular injury. OS 08-03 PHARMACOGENETIC MARKERS OF SURVIVAL Vera Erdman, Ilsia Tuktarova, Timur Nasibullin, Ianina Timasheva, Olga Mustafina. Department of Physiological genetics, Institute of Biochemistry and Genetics, Russia Objective: Physiological and biochemical changes during aging alter drug metabolism. Drug intake is increased with age because of cumulative morbidity, in particular, high prevalence of cardiovascular diseases. Antihypertensive medica- tions are the most commonly used drugs. Individual drug sensitivity or resistance may be influenced by the variance of the “pharmacological response’’ genes. Our purpose was to search for the polymorphic variants of “pharmacological re- sponse’’ genes associated with survival in different age periods. Design and method: The study group consisted of 832 healthy persons aged be- tween 21 and 109 years from the Republic of Bashkortostan (Russia). Participants were divided into three age subgroups: middle-age, elderly and long-livers. Poly- morphic markers of ITGB3 (rs5918), ABCB1 (rs1045642), PTGS1 (rs3842787), PTGS2 (rs20417), F5 (rs6025) and VKORC1 (rs9934438) genes were analyzed by PCR method. Statistical analysis was performed using IBM SPSS V.21.0. Results: ABCB1*T allele was detected with high frequency (58.87%) in the study group, comparable to the majority of Caucasian populations. ABCB1*T allele is associated with increased absorption and slow excretion of xenobiotics, and there- fore testing for this polymorphism is important for selecting medications and dos- age. Similarly high frequency of PTGS2*C allele was observed in the study group (18.63%). PTGS2*C allele is associated with aspirin resistance, which makes it an important pharmacogenetic marker. ITGB3*C allele frequency was decreased in the elderly subgroup compared to the middle-age subgroup (6.44% vs. 17.95%, p = 0.0003). ITGB3*C allele is associated with low response to anticoagulants, and the carriers of ITGB3*C allele have an increased risk of myocardial infarc- tion, stroke, sudden cardiac death, hypertension. Thus, we suggest that the fre- quency of ITGB3*C allele is diminished with age due to negative selection. Conclusions: The results of our study demonstrate the age-related trend for de- creased frequency of polymorphic markers associated with impaired sensitivity to anticoagulants. Supported by RFBR grants No. 14-04-97094_a and 14-04-01169_a. OS 08-04 EPISTATIC AND SEX-DEPENDENT ASSOCIATION ANALYSES OF GENES OF THE RENIN- ANGIOTENSIN-ALDOSTERONE SYSTEM AND BLOOD PRESSURE IN FAMILIES Stephen Harrap 1 , Katrina Scurrah 2 , Angela Lamantia 1 , Justine Ellis 3 . 1 Department of Physiology, University of Melbourne, Australia, 2 Centre for Epidemiology and Biostatistics, University of Melbourne, Australia, 3 Department of Genes, Environ- ment & Complex Disease, Murdoch Childrens Research Institute, Australia Objective: Genes encoding key elements of the renin-angiotensin-aldosterone system (RAAS) cascade have been previously but inconsistently associated with blood pressure. Sex-dependency might be important here and functional genetic polymorphisms might exhibit epistatic effects. Design and method: We assessed variation in the genes encoding renin (REN), angiotensinogen (AGT), angiotensin converting enzyme (ACE), angiotensin II type 1 receptor (AGTR1) and aldosterone synthase (CYP11B2). We determined whether individual SNPs, interactions between SNPs or sex-SNP interactions were associated with systolic blood pressure (SBP). 88 SNPs provided gene-wide coverage of the 5 RAAS genes for 2929 individuals (comprising over 800 2-gen- eration white adult families) from the Victorian Family Heart Study. Variance components models were used that adjusted for measured covariates and within- family correlation. Results: Initial analyses found that three SNPs (rs8075924 and rs4277404 in ACE and rs12721297 in AGTR1) were inversely associated with SBP with significant (p < 0.005) effect sizes around −1.7 mmHg to −2.5 mmHg. No association was observed for markers for well-known polymorphisms: ACE I/D, AGTR1 A1166C and AGT M235T. Significant sex-specific associations were seen for 3 SNPs in males (rs2468523 and rs2478544 in AGT and rs11658531 in ACE) and 1 SNP in females (rs12451328 in ACE). There were significant interactions between 3 pairs of SNPs (rs7079 in AGT with rs2004776 in AGT; rs4736360 in CYP11B with rs2478523 in AGT and rs4736360 in CYP11B with rs2478545 in AGT). The interaction effect estimates ranged from 2.9 to 5.5 mmHg. Conclusions: Our analyses of SNPs in and around key RAAS genes for SBP re- veal evidence of direct association, association dependent on sex and association dependent on epistasis. Variants dependent on epistasis or sex were not obvious in ORAL SESSION 08