www.jss-journal.com Page 1 Journal of Separation Science Received: MONTH DD, YYY; Revised: MONTH DD, YYY; Accepted: MONTH DD, YYY This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as doi: 10.1002/jssc.201600491. This article is protected by copyright. All rights reserved. Optimization and development of a high-performance liquid chromatography method for the simultaneous determination of vitamin E and carotenoids in tomato fruits Maria Irakli 1 , Paschalina Chatzopoulou 1 , Kalliopi Kadoglidou 1 , Nektaria Tsivelika 1 1 Hellenic Agricultural Organization–Demeter, Plant Breeding and Genetic Resources Institute, Thermi, Thessaloniki, Greece Correspondence: Dr. Maria Irakli, Hellenic Agricultural Organization – Demeter, Plant Breeding and Genetic Resources Institute, GR-57001 Thermi, Thessaloniki, Greece E-mail: irakli@cerealinstitute.gr, Fax: +30-2310473024 Running title: Simultaneous HPLC analysis of vitamin E and carotenoids in tomato