Downloaded from www.microbiologyresearch.org by IP: 54.70.40.11 On: Sun, 25 Nov 2018 10:06:22 Streptomyces zagrosensis sp. nov., isolated from soil Fatemeh Mohammadipanah, 1,2 Javad Hamedi, 1,2 Cathrin Spro ¨ er, 3 Manfred Rohde, 4 Marı ´a del Carmen Montero-Calasanz 3 and Hans-Peter Klenk 3 Correspondence Javad Hamedi jhamedi@ut.ac.ir Marı ´a del Carmen Montero- Calasanz maria.montero-calasanz@dsmz.de 1 Department of Microbial Biotechnology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, 14155-6455 Tehran, Iran 2 University of Tehran Microorganisms Collection (UTMC), Microbial Technology and Products Research Center, University of Tehran, Tehran, Iran 3 Leibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstrasse 7b, 38124 Braunschweig, Germany 4 HZI – Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany The taxonomic position of a novel actinomycete isolated from soil in Fars Province (Iran) was determined using a polyphasic approach. Phenotypic characterization and 16S rRNA gene sequence analysis of the isolate matched those described for members of the genus Streptomyces. On ISP2 medium, strain HM 1154 T produced a dark cream, branched substrate mycelium and Retinaculiaperti aerial hyphae that in some images also appeared spiral and that developed into greyish-white spore chains with a smooth surface. The isolate showed optimal growth at 28 6C and pH 6–9 with 0–4 % (w/v) NaCl. Whole-cell hydrolysates contained LL-diaminopimelic acid as diagnostic diamino acid, ribose and glucose. The main phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, three unknown phospholipids and an unknown aminophospholipid; MK-9(H 4 ) and MK-9(H 2 ) were the predominant menaquinones. The major cellular fatty acids were the branched saturated iso-C 16 : 0 and anteiso-C 15 : 0 . Strain HM 1154 T exhibited the highest 16S rRNA gene sequence similarities to Streptomyces coerulescens DSM 40146 T (99.4 %), Streptomyces varsoviensis DSM 40346 T (99.3 %), Streptomyces youssoufiensis DSM 41920 T (99.2 %), Streptomyces abikoensis DSM 40831 T (99.2 %), Streptomyces rimosus subsp. rimosus DSM 40260 T (99.1 %), Streptomyces luteireticuli DSM 40509 T (99.1 %), Streptomyces thioluteus DSM 40027 T (99.1 %), Streptomyces blastmyceticus DSM 40029 T (99.0 %) and Streptomyces hiroshimensis DSM 40037 T (99.0 %). DNA–DNA hybridization studies showed relatedness values of 11.0–35.8 % with the closest related species. Based on these results, strain HM 1154 T is considered to represent a novel species within the genus Streptomyces, for which the name Streptomyces zagrosensis sp. nov. is proposed. The type strain is HM 1154 T (5DSM 42018 T 5UTMC 1154 T 5CECT 8305 T ). The genus Streptomyces was proposed by Waksman & Henrici (1943) (emend. Witt & Stackebrandt, 1990; emend. Wellington et al., 1992) to accommodate aerobic, spore- forming actinomycetes. Members of the genus Streptomyces are Gram-positive bacteria with a DNA rich in G+C content (69–73 mol%) (Williams et al., 1983), contain LL-diaminopimelic acid in the peptidoglycan and lack diagnostic sugars in whole-cell hydrolysates (Lechevalier & Lechevalier, 1970). Species of the genus Streptomyces are common in soil (Zheng et al., 2013; Hamedi et al., 2010; Bouizgarne et al., 2009) and known by their ability to produce biotechnologically useful enzymes (Liu et al., 2013) and most of the biologically active secondary metabolites, such as antibiotics (Kim et al., 2012), anti-tumour agents (Schleissner et al., 2011) and antifungal compounds (Zhao et al., 2010), among others. Peptides, and especially cyclo- peptides, have currently received considerable attention Abbreviations: DPG, diphosphatidylglycerol; ISP, International Strep- tomyces Project; PE, phosphatidylethanolamine; PI, phosphatidylinositol. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain HM 1154 T is JF917242. Two supplementary figures are available with the online version of this paper. International Journal of Systematic and Evolutionary Microbiology (2014), 64, 3434–3440 DOI 10.1099/ijs.0.064527-0 3434 064527 G 2014 IUMS Printed in Great Britain