Abstract—Floating tablets of Marichyadi Vati were developed with an aim to prolong its gastric residence time and increase the bioavailability of drug. Rapid gastrointestinal transit could result in incomplete drug release from the drug delivery system above the absorption zone leading to diminished efficacy of the administered dose. The tablets were prepared by wet granulation technique, using HPMC E50 LV act as Matrixing agent, Carbopol as floating enhancer, microcrystalline cellulose as binder, Sodium bi carbonate as effervescent agent with other excipients. The simplex lattice design was used for selection of variables for tablets formulation. Formulation was optimized on the basis of floating time and in vitro drug release. The results showed that the floating lag time for optimized formulation was found to be 61 second with about 97.32 % of total drug release within 3 hours. The vitro release profiles of drug from the formulation could be best expressed zero order with highest linearity r 2 = 0.9943. It was concluded that the gastroretentive drug delivery system can be developed for Marichyadi Vati containing Piperine to increase the residence time of the drug in the stomach and thereby increasing bioavailability. Keywords—Piperine, Marichyadi Vati, Gastroretentive drug delivery, Floating tablet. I. INTRODUCTION ARICHYADI Vati is one of the best classical formulations mentioned in Ayurvedic formulary of India and widely used in cough and asthma. The daily dose of formulation is 3 Gms as per Ayurvedic Formulary. The Vati contains piper nigrum and piper longum as main ingredients which contain Piperine as major Phyto-constituents. According to pharmacological studies it was reported that Piperine has toxicity in stomach. The principle of buoyant preparations offers a simple and practical approach to achieve increased gastric residence time for the dosage form and sustained drug release. In this study floating drug delivery system (FDDS) is chosen as a method to obtain sufficient bioavailability and maintain therapeutic drug levels. As per the literature it is noted that no one has try to reduce the toxicity of Piperine and it is first attempt to develop NDDs of such Ayurvedic formulation. The aim of the present research study is to develop gastroretentive drug delivery system for Marichyadi Vati using simplex lattice design as an optimization technique [1]. Imran Khan Pathan*, Anil Bhandari, Peeyush K Sharma, Rakesh K Patel, and Suresh Purohit are with the Faculty of Pharmaceutical Sciences, Jodhpur National University 342001, Jodhpur, Rajasthan, India (Corresponding author e-mail: pathanimran15@gmail.com). II. MATERIALS AND METHODS A. Materials Marichyadi Vati was prepared in laboratory and its individual components were procured from, Ms. Sanjivani Ausadhalay, Bhavnagar. The Piperine was obtained from department of Pharmacognosy SKPCPER, Ganpat University, Mehsana, Gujarat, India. B. Methods 1. Preparation of Stock Solution and Determination of Absorption Maxima of Piperine in 0.1N HCl 10mg of Piperine was dissolved in 1ml of methanol and then q.s. to 10ml with 0.1N HCl. 1ml of this solution was further diluted to 10ml in volumetric flask with 0.1N HCl. This was serving as a standard stock solution (100μg/ml). The spectrum of the Piperine was obtained by scanning this solution in the range of 200nm- 400nm against 0.1N HCl as blank to fix absorption maxima. C. Calibration Curve Calibration curve were established with eight dilutions of standard prepared from standard stock solution using further dilution, at concentration range from 2 to 16μg/ml. Each concentration was measured in triplicate. The corresponding absorbance was plotted against the concentration of the marker. The reference substance employed was Piperine. The graph so obtained was shown in Fig. 1. D. Simplex Lattice Design [2], [3], [6]-[8] Simplex lattice design was adopted to optimize the formulation variables. In this design, three factors were evaluated by changing their concentrations simultaneously and keeping their total concentration constant. The simplex lattice design for a 3-component system is represented by an equilateral triangle in 2-dimensional space. Seven batches (S1- S7) were prepared: one at each vertex (A, B, C), one at the halfway point between vertices (AB, BC, AC), and one at the center point (ABC). Each vertex represents a formulation containing the maximum amount of 1 component, with the other 2 components at a minimum level. The halfway point between the 2 vertices represents a formulation containing the average of the minimum and maximum amounts of the 2 ingredients represented by 2 vertices. The center point represents a formulation containing one third of each ingredient. The amounts of matrixing agent (HPMC E50 LV), gas-generating agent (Sodium bicarbonate, X2), and floating enhancer (Ethyl cellulose, X3) were selected as independent variables. The formula was mentioned in Table I. Development and Evaluation of Gastro Retentive Floating Tablets of Ayurvedic Vati Formulation Imran Khan Pathan, Anil Bhandari, Peeyush K. Sharma, Rakesh K. Patel, Suresh Purohit M World Academy of Science, Engineering and Technology International Journal of Pharmacological and Pharmaceutical Sciences Vol:7, No:12, 2013 954 International Scholarly and Scientific Research & Innovation 7(12) 2013 scholar.waset.org/1307-6892/9996907 International Science Index, Pharmacological and Pharmaceutical Sciences Vol:7, No:12, 2013 waset.org/Publication/9996907