_____________________________________________________________________________________________________ *Corresponding author: E-mail: Joachim.nsomue@unikin.ac.cd; International Research Journal of Pure & Applied Chemistry 23(3): 10-18, 2022; Article no.IRJPAC.87870 ISSN: 2231-3443, NLM ID: 101647669 Valorization of Carapa procera Oil and Evaluation in vitro of Antimalarial Activity of Its Bitter Content Muyumba Nsomue Joachim a* , Bolangongo Mpandu Naomie a , Mulula Arnold a , Mbuyi Katshiatshia Haddy b , K. T. Kashishi a , Taba Kalulu a and Joséphine K. Ntumba a a Laboratory of Organic Chemistry and Energy (LACOREN), Department of Chemistry and Industry, University of Kinshasa, Democratic Republic of Congo. b Research Center for Renewable Energy, Faculty of Polytechnic Sciences, University of Kinshasa, Democratic Republic of Congo. Authors’ contributions This work was carried out in collaboration among all authors. Authors MNJ, BMN and KKT designed the study, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Authors KT and JKN managed the analyses of the study. Author MA managed the literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/IRJPAC/2022/v23i330462 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/87870 Received 15 April 2022 Accepted 22 June 2022 Published 02 July 2022 ABSTRACT Aims: To extract the bitter content of Carapa procera oil and to evaluate the antimalarial activity of its bitter content. Study Design: Experimental research. Place and Duration of Study: Department of Chemistry (University of Kinshasa) and National Institute for Biomedical Research, between December 2016 and June 2018. Methodology: The seeds of C. procera were collected in the Kwilu region (Democratic Republic of Congo). A standardized method of Soxhlet was used for the extraction and determination of the oil content. The bitter content of C. procera oil was removed by liquid-liquid extraction. Organoleptic sensory analyzes have used a test of the effectiveness of debittering. The in vitro antimalarial activity of bitter content, extracts, crude oil and the debittered oil on Plasmodium falciparum were evaluated according to the method of maturation test of trophozoites into schizonts. Results: Quality indexes such as acid values (12.72 and 2.72 mg KOH/g), saponification values (190.36 and 193.12 mg KOH/g), peroxide values (5.26 and 19.66 meq O 2 /Kg), and iodine values Original Research Article