*Correspondence: stephens4950@gmail.com; 91+ 9894383368 (Received: 19 January 2019; accepted: 06 March 2019) Citaton: Selvaraj Stephen, Jothimani Pradeep, Balakrishnan Sangeetha and Shanmugam Jayaraman, ‘Seroprevalence of Human Brucellosis in and Around Puducherry: Applicaton of an Immunocapture Agglutnaton Technique (Brucellacapt)’, J Pure Appl Microbiol., 2019; 13(1):313-317 doi: 10.22207/JPAM.13.1.34 © The Author(s) 2019. Open Access. This artcle is distributed under the terms of the Creatve Commons Atributon 4.0 Internatonal License which permits unrestricted use, sharing, distributon, and reproducton in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creatve Commons license, and indicate if changes were made. Stephen et al. J Pure Appl Microbiol, 13(1), 313-317 | March 2019 Artcle 5502 | htps://dx.doi.org/10.22207/JPAM.13.1.34 Print ISSN: 0973-7510; E-ISSN: 2581-690X ReseARCh ARtiCle www.microbiologyjournal.org 313 Journal of Pure and Applied Microbiology seroprevalence of human Brucellosis in and Around Puducherry: Applicaton of an Immunocapture Agglutnaton Technique (Brucellacapt) selvaraj stephen*, Jothimani Pradeep, Balakrishnan sangeetha and shanmugam Jayaraman Department of Microbiology, Mahatma Gandhi Medical College & Research Insttute, Sri Balaji Vidyapeeth (Deemed-to-be-University) Puducherry - 607 402, India. Abstract The objectve of the present study was the serological diagnosis of human brucellosis in and around Puducherry by applicaton of Immunocapture-agglutnaton technique (Brucella Capt), which has been validated and found to be quite satsfactory. The study has included febrile patents, patents with chronic back pain/arthrits, healthy pregnant women, personnel exposed to domestc livestock and voluntary blood donors. Prevalence of brucellosis in these diferent categories was studied. Overall seroprevalence of brucellosis among diferent categories of individuals from Puducherry is very low, viz., 3.27%. Among febrile patents, the prevalence is almost the same with 3.33% positvity. Among 100 healthy pregnant women, only one had antbodies to Brucella abortus (1.00%). The category of animal handlers as expected had higher seroprevalence rate of 8.00%. All the 45 voluntary blood donors were seronegatve for brucellosis. Keywords: Human Brucellosis, Agglutnaton, Brucella abortus, Puducherry.