*Corresponding author: Dr. M. Govindarajan, Assistant Professor, Department of Zoology, Thiru. Vi. Ka. Government Arts College, Tiruvarur 610003, Tamil Nadu, India, Email: drgovind1979@gmail.com, Mobile: +91 9585265999. International Journal of Zoology and Applied Biosciences ISSN: 2455-9571 Volume 2, Issue 3, pp: 128-136, 2017 http://www.ijzab.com https://doi.org/10.5281/zenodo.1311743 Research Article MOSQUITO OVICIDAL AND REPELLENT PROPERTIES OF CHROZOPHORA ROTTLERI (EUPHORBIACEAE) AGAINST ANOPHELES STEPHENSI, AEDES AEGYPTI AND CULEX QUINQUEFASCIATUS (DIPTERA: CULICIDAE) S. Sumithira 1 , A. Amsath 1 , Marimuthu Govindarajan 2* and Udaiyan Muthukumaran 3 1 Department of Zoology, Khadir Mohideen College, Adirampattinam-614701, Tamil Nadu, India. 2 Department of Zoology, Thiru. Vi. Ka. Government Arts College, Tiruvarur-610003, Tamil Nadu, India 3 Unit of Vector Control, Phytochemistry and Nanotechnology, Department of Zoology, Annamalai University, Annamalainagar-608 002, Tamil Nadu, India Article History: Received 18 th March 2017; Accepted 1 st June 2017; Published 26 th June 2017 ABSTRACT Present study, we evaluated the larvicidal and repellent activities of Chrozophora rottleri (Family: Euphorbiaceae) extract against Culex quinquefasciatus, Aedes aegypti and Anopheles stephensi. The ovicidal activity was determined against three mosquito species to various concentrations ranging from 50-300 ppm under the laboratory conditions. The hatch rates were assessed 48 h post treatment. The repellent efficacy was determined against three mosquito species at three concentrations viz., 1.0, 2.5 and 5.0 mg/cm 2 under the laboratory conditions. Among five solvent extracts tested, the methanol extract have most promising ovicidal activity. The methanol extract exerted zero hatchability (100% mortality) at 150 ppm for Cx. quinquefasciatus, Ae. aegypti and An. stephensi. The methanol extract of C. rottleri found to be more repellency than the other extracts. A higher concentration of 5.0 mg/cm 2 provided 100% protection up to 300 min against Cx. quinquefasciatus and 250 min against Ae. aegypti and An. stephensi, respectively. The results clearly show that repellent activity was dose dependent. From the results it can be concluded the crude extract of C. rottleri was an excellent potential for controlling Cx. quinquefasciatus, Ae. aegypti and An. stephensi mosquitoes. Keywords: Chrozophora rottleri, Ovicidal activity, Repellent activity, Culex quinquefasciatus, Aedes aegypti, Anopheles stephensi. INTRODUCTION Mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. Mosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis, and Japanese encephalitis. Mosquitoes also cause allergic responses in humans that include local skin and systemic reactions such as angioedema (Peng et al., 1999). Anopheles stephensi are major malaria vectors in India. With an annual incidence of 300-500 million clinically manifested cases and a death toll of 1.1-2.7 million, malaria is still one of the most important communicable diseases. Currently, about 40% of the world’s population lives in areas where malaria is endemic (Wernsdorfer and Wernsdorfer, 2003). Aedes aegypti is generally known as a vector for an arbovirus responsible for dengue fever, which is endemic to Southeast Asia, the Pacific island area, Africa, and the Americas. This mosquito is also the vector of yellow fever in Central and South America and West Africa. Dengue fever has become an important public health problem as the number of reported cases continues to increase, especially with more severe forms of the disease, dengue haemorrhagic fever and dengue shock syndrome, or with unusual manifestations