Research Article Inhibition of α-Amylase and α-Glucosidase of Anthocyanin Isolated from Berberis integerrima Bunge Fruits: A Model of Antidiabetic Compounds Soheila Moein , 1 Mahmoodreza Moein , 1,2 and Hassan Javid 3 1 Medicinal Plants Processing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran 2 Department of Pharmacognosy, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran 3 BiochemistryDepartment, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandarabbas, Iran Correspondence should be addressed to Soheila Moein; soheila_9@yahoo.com Received 10 November 2021; Accepted 14 February 2022; Published 7 March 2022 Academic Editor: Hicham Harhar Copyright©2022SoheilaMoeinetal.isisanopenaccessarticledistributedundertheCreativeCommonsAttributionLicense, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Anthocyaninsarecomponentsoftheflavonoidgroupwithdifferentproperties,suchasantidiabeticproperties.isstudyaimed to isolate anthocyanin from Berberis integerrima Bunge fruits and evaluate α-amylase and α-glucosidase inhibition by this mentioned anthocyanin. e anthocyanin of Berberis integerrima fruit was isolated using column chromatography, and the antidiabeticpropertiesoftheanthocyaninweredeterminedbythelevelsof α-amylaseand α-glucosidaseinhibition.KmandVmax werealsoevaluatedusingtheGraphPadPrism7.eresultsofthisstudyshowedthattheanthocyanincontentofthefruitextract was 14.36 ± 0.33mg/g, and following purification, this amount increased to 34.51 ± 0.42mg/g. e highest of α-glucosidase inhibition was observed in the purified anthocyanin with IC 50 � 0.71 ± 0.085mg/ml, compared to acarbose as the baseline with IC 50 � 8.8 ± 0.14mg/ml, p < 0.0001. Purified anthocyanin of the mentioned fruit with IC 50 � 1.14 ± 0.003mg/ml had the greatest α-amylase inhibition, which was similar to acarbose as the standard with IC 50 � 1 ± 0.085mg/ml, p < 0.05. e inhibition of α-glucosidaseand α-amylasebypurifiedanthocyaninshoweduncompetitiveinhibition,andtheenzymeinhibitionbyunpurified anthocyaninshowedmixedinhibition.eobtainedfindingsshowedthat Berberis integerrima fruitcanbementionedasasource of anthocyanin with antidiabetic properties. 1. Introduction e characteristics of the metabolic syndrome such as di- abetesincludehighlevelsofglucose,obesityoftheabdomen, andhighlevelofcholesterolandhighbloodpressure,which increasetheriskofcardiovasculardisease.Insulinresistance is a significant feature of metabolic syndrome and type 2 diabetes.Nowadays,thereare246millionpatientswithtype 2 diabetes in the world. e International Diabetes Feder- ation (IDF) announced that 380 million people will be di- abeticin2025[1].Someplantextractsandplantcompounds can reduce blood sugar and prevent diabetes. In folk medicine, barberry fruits are consumed as an antidiabetic drug [2], which composed of great amounts of anthocyanin [3]. Anthocyanin pigments are globally well- known as medicinal agents. Free radical scavengers are the most widely known properties of anthocyanins [3]. Inaddition,itwasreportedthattheanthocyanincontentof barberry fruits is similar to the anthocyanin content of black- berries fruits (Rubus glaucus) [4], nasturtium (Tropaeolum majus)[5],andahybridofstrawberries(Fragaria ananassa)[6]. Some studies [7] showed that, in vitro, anthocyanins stimulate insulin secretion from the rodent pancreatic β-cells.Besides,consumptionoffruitsandvegetablesrichin polyphenols reduces the incidence of type 2 diabetes [8, 9]. Anthocyanin compounds are abundant in food plants and appear in 27 plant families. e usage of anthocyanin hasbeenestimatedat10000tonsofblackgrapesinoneyear. Anthocyanins are vital components of plant secondary metabolites and are also the most important coloring sub- stances in wine [10]. Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2022, Article ID 6529590, 6 pages https://doi.org/10.1155/2022/6529590