46 Cellular and Molecular Biology Original Research Investigation of biological effects of some Mannich Bases containing Bis-1,2,4- Triazole A. E. Parlak 1* , S. Celik 2 , M. Karatepe 3 , S. Turkoglu 4 , N. O. Alayunt 5 , S. D. Dastan 6 , M. Ulas 7 , S. Sandal 8 , S. Tekin 8 , M. Koparir 3 1 Firat University, Keban Vocational High School, 23740, Elazig, Turkey 2 Usak University, Faculty of Dentistry, 64200, Usak, Turkey 3 Firat University, Faculty of Science, Department of Chemistry, 23119, Elazig, Turkey 4 Fırat University, Faculty of Health Sciences, 23119, Elazig, Turkey 5 Usak University, Banaz Vocational High School, 64200, Usak, Turkey 6 Cumhuriyet University, Faculty of Veterinary Medicine, Department of Biometrics and Genetics, 58140, Sivas, Turkey 7 Firat University, Faculty of Medicine, Department of Physiology, 23119, Elazig, Turkey 8 Inonu University, Faculty of Medicine, Department of Physiology, 44000, Malatya, Turkey Abstract: In this study, the effects of Mannich bases containing bis-1,2,4-triazole on the levels of in vivo malondialdehyde (MDA) and an- tioxidant vitamins (A, E, C) were examined in serum, livers and kidneys of rats. DA and vitamin (A, E, C) levels were determined by high performance liquid chromatography (HPLC). Antioxidant effect was investigated by determining the MDA levels in Saccharomyces cerevisiae cells as in vitro. Furthermore, the antitumor effects of compounds were investigated against MCF-7 human breast cancer cells. Interrelations of results among control and compound groups were evaluated using SPSS statistical software package. As a result, some of the compounds showed effective biological activity when compared to control conditions. The test compounds used in this study may be effective for utilization in the selection and design of model compounds for further studies. Key words: Mannich bases, 1,2,4-triazole, Biological activity. Introduction Having potential in biology, Mannich bases have found quite a large application area in medicine in re- cent years. Nowadays, there is an increasing interest in heterocyclic molecules containing nitrogen because of their intense range of biological activity. Intensive stud- ies have been carried out on these compounds by many research groups because of quite remarkable biological characters of triazole compounds (1, 2). Various bio- logical activities like antineoplastic (3, 4), antimalarial (5) and antiviral (6) effects of Mannich bases have been reported. One of the methods that have become commonly used in the study of drug designs is a set of Mannich reactions that lead to the synthesis of organic molecules in a single step containing different functional groups in a single molecular structure. Mannich bases, with high water solubility, are chemical structures preferred in preparing prodrugs (7, 8). Pharmaceutical chemistry is the most important usage area of Mannich bases, and 35% of the publications in this area are about Mannich bases. In pharmaceutical chemistry; they are used in an- algesics, antibiotics and anti-cancer drugs (9). Triazoles were introduced to the scientifc world by Bladin (10), and studies of Andreocci in the 1880s (11) on this sub- ject have been taken intensively further until today. Tri- azoles and thiadiazole constitute a practical set of sub- jects with their chemical activities suitable to be placed on the structure of various substituents, and especially their tautomeric properties (12, 13). In the studies per- formed with triazole derivatives in the literature, effec- tive compounds of antimicrobial (14), virostatic (15), cytostatic (16), anti-infammatory (17), analgesic (18), anticonvulsant (19), central nervous system depressant (20), antihistaminic (21), hypotensive (22), diuretic (23), anthelmintic (24), antifungal (25), pesticide (26) and insecticide (27) properties have been found. The fact that both triazoles and Mannich bases are biologically active compounds has led to the idea of synthesizing Mannich bases over 1, 2, 4 - triazoles, and caused these compounds to gain a wide range of appli- cations in pharmaceutical chemistry. This study aimed to contribute to other works on this issue by searching the bioactivity of some Mannich bases containing bis- 1, 2, 4 - triazoles. Towards this aim in this study, anti- oxidant properties were investigated with MDA assay through synthesizing some Mannich bases containing bis-1, 2, 4 – triazoles and by using an in vitro method in Saccharomyces cerevisiae cells. Saccharomyces cerevi- siae, which belongs to the kingdom of fungi, is a uni- cellular microorganism. Saccharomyces cerevisiae is an ascomycete yeast. This type of yeast has dominantly a unicellular talus that produces ascospores in a free as- cus that is composed of a zygote or a single somatic cell as a parthenogenetic, and it reproduces asexually with methods like budding and transverse division. Saccharomyces cerevisiae ferments carbohydrates like other yeasts. It is used in beer production and bakery. High vitamin content of yeasts increases their value as food ingredients. Many types of Saccharomyces cerevi- Received February 18, 2016; Accepted May 31, 2016; Published June 30, 2016 * Corresponding author: A. E. Parlak, Firat University, Keban Vocational High School, 23740, Elazig, Turkey. Email: akifparlak23@gmail.com Copyright: © 2016 by the C.M.B. Association. All rights reserved. Parlak et al. Cell. Mol. Biol.2016, 62 (7): 46-54 ISSN: 1165-158X doi: 10.14715/cmb/2016.62.7.9