UDC 615.628.1 DOI: 10.48077/scihor.24(9).2021.9-18 Determination of Toxicity of Experimental Disinfectant Tetyana Fotina, Oksana Shkromada * , Hanna Fotina, Andriy Berezovsky, Daria Slasten Sumy National Agrarian University 40021, 160 H. Kondratiev Str., Sumy, Ukraine Abstract. Excessive use and abuse of disinfectants over the past century has created problems associated with the emergence of resistant microorganisms. In addition, there is always a potential risk to human and animal health, as the use of aggressive disinfectants can lead to diseases. The purpose of the study was to investigate the toxicity of an ex perimental disinfectant to determine the possibility of its use in the presence of humans and animals. Study material – an experimental product (water-soluble disinfection powder). Experimental studies were conducted at the premises of a certifed vivarium of the ECOMEDCHIM regional centre of Sumy State University and Sumy National Agrarian University. Acute toxicity of the preparation was studied on 30 white mice weighing 19.5±1.0 and 15 white rats weighing 200±15.0 g. Determination of toxicity by prolonged oral administration was studied in two analogous groups of white rats, 6 animals each with a bodyweight of 185±10.0 g. The effect of the preparation on the state of internal organs was evaluated by comparing relative mass coeffcients. The local irritating effect of the preparation was determined by skin applications on 10 rabbits weighing 2.97±0.3 kg. The disinfectant, when administered orally once at doses of 1,250, 2,500, and 5,000 mg/kg of body weight, did not cause the death of experimental mice and rats. When administered orally for 30 days at a dose of 2,500 mg/kg of body weight, the disinfectant did not cause any negative and toxic effects on the body of experimental rats, did not affect the growth and development of rats, and did not cause changes in the relative mass of internal organs. It was found that the average manifestation of erythema in rabbits is 2.46. According to the study results, it was found that the disinfectant can be classifed as Hazard Class 4 according to the international standard GOST 12.1.007-76, or Category 5 according to the international global classifcation Global Harmonised System (GHS) (LD 50 with oral administration exceeds 5,000 mg/kg of body weight). The results obtained give grounds to assert the possibility of using an experimental disinfectant in the presence of humans and animals Keywords: disinfectant, acute toxicity, skin-resorptive effect, laboratory animals, erythema, oedema Article’s History: Received: 23.09.2021 Revised: 21.10.2021 Accepted: 22.11.2021 Suggested Citation: Fotina, T., Shkromada, O., Fotina, H., Berezovsky, A., & Slastyon, D. (2021). Determination of toxicity of experi- mental disinfectant. Scientifc Horizons, 24(9), 9-18. SCIENTIFIC HORIZONS Journal homepage: https://sciencehorizon.com.ua Scientifc Horizons, 24(9), 9-18 Copyright © The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License 4.0 (https://creativecommons.org/licenses/by/4.0/) *Corresponding author