Journal of Animal Research: v.10 n.6, p. 1037-1042. December 2020 DOI: 10.30954/2277-940X.06.2020.24 How to cite this article: Manoj, J. and Singh, M.K. (2020). Seasonal Prevalence and Antibiogram Studies of Bovine Mastitis in Southern Haryana. J. Anim. Res., 10(6): 1037-1042. Source of Support: None; Conflict of Interest: None Seasonal Prevalence and Antibiogram Studies of Bovine Mastitis in Southern Haryana Jinu Manoj 1* and Manoj Kumar Singh 2 1 College Central Laboratory, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, Haryana, INDIA 2 Department of Livestock Production and Management, College of Veterinary and Animal Sciences, SVPUAT, Meerut, U.P., INDIA *Corresponding author: J Manoj; E-mail: drjinumanoj@gmail.com Received: 17 Sept., 2020 Revised: 26 Nov., 2020 Accepted: 10 Dec., 2020 ABSTRACT Mastitis in bovines causes huge economical loss to the dairy industry. It affects the public health due to the transmission of pathogenic bacteria and the toxins produced by them through the food chain. The timely diagnosis of subclinical mastitis (SCM) and its proper treatment based on antibiogram have signifcant impact on reducing the economical loss to the dairy farmers. Therefore the present study was conducted to fnd out the prevalence and antibiogram pattern of the mastitis causing bacteria in Southern Haryana. A total prevalence rate of 65.79% was observed for subclinical mastitis in Southern Haryana by the screening test. The highest prevalence was observed in winter (59.49%), followed by rainy season (69.61%) and cattle were found as more susceptible to SCM when compared to buffaloes. The major Gram negative pathogens isolated were belonged to E. coli, followed by Klebsiella species and Pseudomonas species, the common organisms responsible for environmental mastitis. The antibiogram patterns of the isolates were also studied and the maximum sensitive antibiotic against mastitis was found as Chloramphenicol, followed by Enrofoxacin, Amikacin and Ampicillin. The most resistant antibiotics in the study area was found as Ceftizoxime followed by Amoxicillin as these antibiotics were frequently and indiscriminately used for the treatment of mastitis in the study area without subjecting to in vitro antibiotic sensitive test. This emphasis the need of awareness among dairy farmers regarding the hygienic managemental practices to be followed at farm level as well as necessity of conducting antibiotic sensitivity test for the treatment. HIGHLIGHTS m A total prevalence of mastitis observed was 65.79%. m The most resistant antibiotics were Ceftizoxime and Amoxicillin. m The most sensitive antibiotics were Chloramphenicol and Enrofoxacin. Keywords: Antibiogram, Haryana, Mastitis, Prevalence, Seasonal Mastitis the potentially fatal udder tissue infection is the most common disease in dairy animals. It usually occurs due to the infammatory response to bacterial invasion of the teat canal. It can also occur as a result of chemical, mechanical or thermal injury to the udder (Akhoon et al., 2015). It affects the public health due to the transmission of pathogenic bacteria and their toxins through the food chain (Argaw, 2016; Shaheen et al., 2016; Hameed et al., 2007). The prevalence of subclinical mastitis (SCM) is more prevalent than clinical mastitis (Sharma et al., 2012). Meta analysis revealed 29-45% increased prevalence of subclinical mastitis during 2011 to 2016 (Krishnamoorthy et al., 2017). The subclinical mastitis lacks visible infammatory changes initially and for the