respectively). No differences were observed in body mass index (BMI) or waist circumference, while C3 levels were significantly increased in MetS subjects (1.65±0.28 g/L and 1.56±0.24 g/L, respectively). C3 showed the strongest correlations with BMI (r¼0.246, p<0.001) and insulin (r¼0.248, p<0.001). After multi-factor adjustment, the OR for MetS for each C3 in- crease of 1g/L was4.09 (1.42-11.76), p¼0.009. Conclusions: In morbid obesity, the level of C3 can differentiate between subjects with and without MetS. This marker may be a useful tool to identify morbidly obese subjects with an increased risk for MetS and probably increased cardiovascular risk. PO294. NEUTROPHIL TO LYMPHOCYTE RATIO AND ABDOMINAL AORTIC ATHEROSCLEROSIS AMONG ASYMPTOMATIC INDIVIDUALS Carolina Stangenhaus, Antonio Gabriele Laurinavicius , Fernando H.Y. Cesena, Raquel Conceicao, Raul D. Santos, Marcio Sommer Bittencourt. The Hospital Israelita Albert Einstein, Sao Paulo, Brazil Aim: Neutrophil-lymphocyte ratio (NLR) was proposed as an inflamma- tory marker of coronary atherosclerosis, though its association with extra- coronary atherosclerosis remains unclear. We analysed the association between NLR and abdominal aortic atherosclerosis (AAAt). Methods: We included asymptomatic individuals who underwent a health-screening program. AAAt was determined by ultrasound. Leuko- cytes and lymphocytes absolute values were used to estimate the NLR. Results: Among 36,985 individuals (age: 42±10 years; 72% male) AAAt was identified in 7%. Those with AAAt were older, more likely to be male and diabetic. Prevalence of AAAt was associated with NLR (OR 1.17; CI: 1.13 - 1.21). However, the association was lost when adjusted for risk factors (OR 1.02; CI: 0.97 - 1.06), mostly due to the inclusion of age in the model. When exploring the neutrophils and lymphocytes separately, the negative as- sociation between lymphocytes and AAAt was inverted once accounting for age; suggesting a strong confounding effect of age on lymphocytes (and NLR), as absolute values of lymphocytes decreased with aging. Finally, the association of absolute neutrophils and AAAt lost significance after addi- tional adjustment for traditional risk factors, but not age alone. Conclusions: Although the NLR is associated with AAAt, this is largely due to the confounding effect of age. The association of neutrophils and lym- phocytes with AAAt is likely an inflammatory mediation of the effect of risk factors on AAAt development, and loses its significance once risk factors are included in multivariable models. Overall, these results suggest a limited role of leukocyte measurements as biomarkers of AAAt. PO295. LIPOPROTEIN PROFILING WITH THE AXINON® SYSTEM Daniela Baumstark 1 , Sindy Neumann 1 , Johannes Eiglsperger 1 , Sabine Norkauer 1 , Jakob Wieczorek 1 , Markus Fuhrmann 1 , Michael Mündner 1 , Julia Wack 1 , Hubert Scharnagl 2 , Tatjana Stojakovic 2 , Winfried M€ arz 3 , Philipp Pagel 1 . 1 Numares AG, Regensburg, Germany; 2 Medical University of Graz, Graz, Austria; 3 Medical Clinic V, Mannheim, Germany Aim: To date, the widespread application of lipoprotein profiling has been hindered by the laborious and time-consuming nature of existing methods. By means of the NMR-based, CE-marked AXINON® lipoFIT®- S100 test system lipoprotein profiling became available for in vitro diag- nostic and research use. AXINON® lipoFIT®-S100 is intended for quanti- fication of lipoprotein particle and cholesterol concentrations in lipoprotein subclasses, mean particle sizes as well as the concentrations of total cholesterol, triglycerides, LDL-C, HDL-C and several metabolites in human serum. Now a new test allowing a more detailed analysis of lipo- protein composition is available for research use. This test measures over 40 parameters such as the triglyceride, total cholesterol, and phospholipid concentrations in the lipoprotein subclasses. This study aimed at evaluating the performance characteristics of the new test against lipoFIT®-S100. Methods: Assay performance was evaluated based on Clinical and Labo- ratory Standards Institute (CLSI) guidelines. Results: The performance characteristics in terms of linearity, precision, trueness, interfering substances, matrix effects and throughput will be presented. Both test systems showed very good correlation in method comparison. For AXINON® lipoFIT®-S100, the mean total imprecision between three different systems was 3.7%, demonstrating an overall excellent precision. Conclusions: The analytical performance characteristics of both tests underline that they are both fit for routine use, especially for studies with a large number of samples. The growing numares' test portfolio makes AXINON® an attractive lab solution for metabolomics in laboratory di- agnostics and biomarker research. PO296. CREATION OF CYBRID CULTURES CONTAINING MITOCHONDRIAL GENOME MUTATION M.12315G>A, ASSOCIATED WITH ATHEROSCLEROSIS V. Vasily Sinyov 1 , A. Margarita Sazonova 1, 2 , I. Anastasia Ryzhkova 2 , V. Elena Galitsyna 6 , V. Andrey Zhelankin 1 , Y. Konstantin Mitrofanov 2 , V. Yuri Bobryshev 2, 3,4 , N. Alexander Orekhov 2, 5 , A. Igor Sobenin 1, 2 . 1 Russian Cardiology Research and Production Complex, Laboratory of Medical Genetics, Moscow, Russia; 2 Institute of General Pathology and Pathophysiology, Laboratory of Cellular Mechanisms of Atherogenesis, Moscow, Russia; 3 University of New South Wales, Faculty of Medicine, School of Medical Sciences, Sydney, Australia; 4 University of Western Sydney, School of Medicine, Campbelltown, NSW, Australia; 5 Skolkovo Innovative Centre, Institute for Atherosclerosis Research, Moscow Region, Russia; 6 Southern Federal University, Department of Genetics, Rostov-on- Don, Russia Aim: The aim of the present study was a creation of cybrid cultures con- taining mitochondrial genome mutation m.12315G>A, associated with atherosclerosis. Methods: During the conduction of this research work rho0-cell line created on the basis of monocytic origin culture THP-1 was used. Cybrid cultures were obtained by PEG-fusion of rho0-cells and platelets of patients. The criterion for selection of platelet donors was the threshold heteroplasmy level of mitochondrial mutation m.12315G>A, associated with atherosclerotic lesions in human [1]. Results: Two cybrid cell cultures were obtained, in which the hetero- plasmy level of mitochondrial genome mutation m.12315G>A exceeded the threshold value in atherosclerotic plaques (7,5%) and in thickened in- tima-medial layer of the carotid arteries (10,5%) [1]. In the first cybrid line the heteroplasmy level of mutation m.12315G> A was 25%, and in the second cybrid line it was 44%. Conclusions: In the present study cybrid cell cultures containing mito- chondrial genome mutation m.12315G>A, associated with atherosclerosis were created. These cybrids can be models for investigating the mecha- nisms of atherogenesis and mitochondrial dysfunction and for the devel- opment of approaches to the treatment of atherosclerosis. The article can be useful for specialists in cell biology, medical genetics, and doctors specializing in cardiovascular pathologies. This study was supported by Russian Scientific Foundation, grant #14-14- 01038. Reference 1. Sazonova M.A., Orekhov A.N., Sobenin I.A. Mitochondrial genome defects and atherosclerosis. Role of mitochondrial genome pathologies in atherosclerotic lesions formation of an arterial wall. Palmarium Academic Publishing, 2014. e 354p. ISBN: 978-3-639-88097-7. Russian. PO297. MULTI-MARKER EVALUATION OF THE CARDIO-METABOLIC RISK Mihaela Mocan 1 , Rodica Rahaian 1 , Bogdan Mocan 2 , Sorin Nicu Blaga 1 . 1 University of Medicine and Pharmacy Iuliu Hatieganu, Cluj-Napoca, Romania; 2 Technical University, Cluj-Napoca, Romania Abstracts / Atherosclerosis 263 (2017) e111ee282 e201