Emir. J. Food Agric. 2010. 22 (3): 223-231 http://ffa.uaeu.ac.ae/ejfa.shtml 223 Short Communication A fast and efficient method to determine the position of alien genes in transgenic plants Mahmood-ur-Rahman, S. Noreen, T. Husnain ∗ and S. Riazuddin National Centre of Excellence in Molecular Biology (CEMB), 87-West Canal Bank Road, Thokar Niaz Baig, Lahore-53700, Pakistan Abstract: Phenotypic variations are commonly observed in transgenic plants. Basmati rice (Oryza sativa L.) and cotton (Gossypium hirsutum), locally transformed with Bt genes, also exhibited such variations in their morphology. One possible cause of these variations might be the position of transgene on chromosome (s). An experiment was conducted to determine the position of the alien genes. It was determined by Fluorescence in situ hybridization (FISH) by hybridizing fluorescien labeled DNA probe on mitotic chromosomes of transgenic rice and cotton. An easy and simple protocol was developed as compared to available lengthy and difficult methods. The method is quick and efficient. Keywords: Bt genes, Fluorescence in situ hybridization, transgene, DNA probe. وراﺛﻴﺎ اﻟﻤﻌﺪﻟﺔ اﻟﻨﺒﺎﺗﺎت ﻓﻰ اﻟﻐﺮﻳﺒﺔ اﻟﺠﻴﻨﺎت اﻣﺎآﻦ ﻟﺘﺤﺪﻳﺪ ﺗﺰﻳﺪ وﻓﻌﺎﻟﺔ ﺳﺮﻳﻌﺔ ﻃﺮق ﻣﺤﻤﻮد– ار- رﺣﻤ ﺎ ن, س. ت ﻧﻮرﻳﻦ،. ﺣﺴﻨﻴﻦ * س و. رﻳﺎزودﻳﻦ اﻟﺠﺰﻳﺌﺔ اﻟﺒﻴﻠﻮﺟﻴﺎ ﻓﻰ ﻟﻠﺘﻔﻮق اﻟﻮﻃﻨﻰ اﻟﻤﺮآﺰCEMB ، 87 ﺷﺎرع، ﻟﺸﺎر اﻟﻐﺮﺑﻴﺔ اﻟﻀﻔﺔ ا ع ﻻهﻮر، ﻧﺎﻳﺰﺑﻴﻖ، ﺳﻮآﺎر ﻟﻘﻨﺎة،35700 ﺑﺎآﺴﺘﺎن، اﻟﻤﻠﺨﺺ: ﻋﺎدة ﻧﻼﺣﻆ وﺟﻮد اﻟﻤﻈﻬ اﻻﺧﺘﻼﻓﺎت وﺧﺎﺻﺔ وراﺛﻴﺎ اﻟﻤﻌﺪﻟﺔ اﻟﻨﺒﺎﺗﺎت ﻓﻲ ﺮﻳﺔ واﻟﺬي واﻟﻘﻄﻦ اﻟﺒﺴﻤﺘﻰ اﻷرز ﻧﺒﺎت ﻓﻲ ﺗﻲ ﺑﻲ ﺟﻴﻨﺎت ﺗﻘﻨﻴﺔ ﺑﻮاﺳﻄﺔ ﻣﺤﻠﻴﺎ ﺗﺤﻮل ﻟﻬﺬﻩ اﻷﺳﺒﺎب أهﻢ وﻣﻦ اﻟﺨﺎرﺟﻲ ﺷﻜﻠﻬﺎ ﻓﻲ وﺗﻨﻮع اﺧﺘﻼﻓﺎت اﻇﻬﺮ ﻣﻤﺎ اﻻﺧﺘﻼﻓﺎت اﻟﻜﺮوﻣﻮﺳﻮم ﻋﻠﻲ اﻟﻤﺤﻮرة اﻟﺠﻴﻨﺎت ﻣﻮﻗﻊ هﻮ واﻟﺘﻐﻴﺮات ﺗﻢ وﻗﺪ اﻟﻐﺮﻳﺒﺔ اﻟﺠﻴﻨﺎت هﺬﻩ ﻣﻮﻗﻊ ﻟﺘﺤﺪﻳﺪ اﻟﺘﺠﺮﺑﺔ هﺬﻩ إﺟﺮاء ﺗﻢ وﻗﺪ ﺗﻘﻨﻴﺔ ﺑﻮاﺳﻄﺔ ﺗﺤﺪﻳﺪهﺎFluorescence in situ hybridization (FISH) اﻟﻤﻌﻠﻢ اﻟﻨﻮوي اﻟﺤﻤﺾ ﺗﻬﺠﻴﻦ ﺧﻼل وﻣﻦ ﺑ ﺎﻟﻔﻠﻮروﺳﻴﻦ اﻟﻜﺮ ﺑﻮاﺳﻄﺔ واﻟﻤﺤﻘﻖ ﻋﻠﻰ اﻟﺘﺠﺮﺑﺔ ﻧﻬﺎﻳﺔ ﻓﻲ اﻟﺤﺼﻮل ﺗﻢ وﻗﺪ وراﺛﻴﺎ اﻟﻤﻌﺪل واﻟﻘﻄﻦ اﻷرز ﻧﺒﺎت ﻣﻦ وﻣﻮﺳﻮﻣﺎت ﻓﺈﻧﻨﺎ وﻋﻠﻴﻪ واﻟﺼﻌﻮﺑﺔ ﺑﺎﻟﻄﻮل ﺗﺘﻤﻴﺰ واﻟﺘﻲ ﺣﺎﻟﻴﺎ اﻟﻤﺴﺘﺨﺪﻣﺔ اﻟﺒﺮوﺗﻮآﻮﻻت ﻣﻊ ﻣﻘﺎرﻧﺔ وﻣﺘﻘﺪم وﺑﺴﻴﻂ ﺳﻬﻞ ﺑﺮوﺗﻮآﻮل ﻃﻮرﻧﺎ هﺬﻩ اﻟﺘﺠﺮﺑﺔ هﺬﻩ ﻓﻲ اﻟﻤﺴﺘﺨﺪﻣﺔ اﻟﻄﺮﻳﻘﺔ و وﻓﻌﺎﻟﺔ ﺳﺮﻳﻌﺔ آﺎﻧﺖ ∗ Corresponding Author, Email: tayyabhusnain@gmail.com