Ximenis,, VD et al. (2022). Jurnal Biologi Tropis, 22 (2): 461 – 470 DOI: http://dx.doi.org/10.29303/jbt.v22i2.3351 461 Original Research Paper The Activity of Lenglengan Leaf Extract (Leucas lavandulifolia Sm.) as an Antibacterial for Staphylococcus aureus Virgolie D. Ximenis 1* , Refli 1 , Djeffry Amalo 1 , Alfred Dima 1 , Rony Mauboy 1 , Maria Ruma 1 1 Program Study Biologi, FST Universitas Nusa Cendana, Kupang, Indonesia. Article History Received : February 28 th , 2022 Revised : March 20 th , 2022 Accepted : April 10 th , 2022 *Corresponding Author: Virgolie D. Ximenis 1 , Program Studi Biologi, Fakultas Sains dan Teknik, Universitas Nusa Cendana, Kupang, Indonesia. Email: virgisimenes@gmail.com Abstract: Dafala village is one of the villages in NTT that uses lenglengan leaves as traditional medicine to treat diseases such as coughs, TBC, diarrhea and back pain.This present research aims to know the bioactive compounds and composition of the bioactive contained in Lenglengan (Leucas lavandulifolia Sm.) leaf extract, and too know the effectiveness of lenglengan leaf extract in inhibiting Staphyloccocus areus bacteria. We extracted leaves of Lenglengan by maceration method using ethanol. The extract was obtained and then tested in a qualitative way to determine the bioactive compounds are made by using reagents. While in the quantitative test to Determine of levels of flavonoids, phenols and tannins using UV-Vis spectrophotometry method, alkaloids and saponins using the gravimetric method. The Antibacterial test was carried out by disc paper method. Paper discs containing extract concentrations: 12.5%, 25%, 50%, 62.5% and 75%, positive control (gentamicin) and negative control (aquadest) were placed on MHA media containing S. aureus suspension, incubated at 37C for 24 hours and then the diameter of the inhibition was observed. Qualititive test result show that the lenglengan leaf extract contains 5 bioactive compounds include alkaloids, flavonoids, saponins, tannins, and phenols. The composition of bioactive compounds in Lenglengan leaf extract: flavonoids 23.93%, saponins: 19.05%, alkaloids: 15.28%, tannins: 5.81%, and phenols: 2,335%. The atibacterial result reveal that lenglengan leaf extract was significantly affect in inhibiting Staphylococcus aureus. The higher concentration of extract lenglengan leaves used, the higher the inhibition zone produced. The highest level response inhibition showed from level of 12.5%: 11.3 mm, 25%: 12.4 mm, 50%: 13.43 mm, 62.5%: 14.43 mm and 75%: 15.58 mm, gentamicin: 17.4 mm aquades: 0 mm. Keywords: Antibacterial, bioactive, Lenglengan, Staphylococcus aureus. Pendahuluan Bakteri patogen merupakan salah satu penyebab penyakit infeksi di daerah beriklim tropis seperti Indonesia (Sari et al, 2017). Hal ini dikarenakan daerah beriklim tropis memiliki udara yang hangat dan lembab serta sanitasi yang buruk sehingga memudahkan penyebaran penyakit infeksi. Penyakit yang disebabkan oleh infeksi merupakan salah satu penyebab kematian yang cukup tinggi di Indonesia. Infeksi yang sering dialami oleh masyarakat antara lain infeksi kulit seperti bisul, luka bakar, luka karena gigitan ular, infeksi saluran pernafasan seperti batuk, TBC, asma, dll (Sumiyati, 2014). Penyebab penyakit infeksi tersebut adalah Staphylococcus aureus. Bakteri ini dapat hidup sebagai saprofit pada permukaan kulit, saluran usus, kelenjar keringat dan saluran tubuh manusia (Ningsih et al, 2013) disertai peradangan ringan hingga akut. Upaya pencegahan (preventive) dan pengobatan (kuratif) sudah dilakukan dengan pendekatan medis dan penggunaan herbal alami. Pendekatan medis dilakukan dengan pemberian antibiotik. Namun penggunaan antibiotik yang semakin meluas disertai ketidakdisiplinan dan atau ketidaktahuan masyarakat menyebabkan peningkatan resistensi. Peningkatan resistensi bakteri menjadi pernasalahan tetap tingginya prevalensi penyakit infeksi pada masyarakat jika tidak diimbangi penemuan antibiotik baru. Oleh karena itu, penggunaan pendekatan herbal alami menjadi salah satu alternatif untuk menghambat Jurnal Biologi Tropis This article is licensed under a Creative Commons Attribution 4.0 International License. © 2022 The Author(s). This article is open access