_____________________________________________________________________________________________________ *Corresponding author: E-mail: bukola.ajide@binghamuni.edu.ng, bukze2000@yahoo.com; Asian Journal of Immunology 4(4): 36-46, 2020; Article no.AJI.64335 Prevalence of Rotavirus in Children with Diarrhea Attending Hospitals in Nasarawa State as a Case Study B. A. Ajide 1* , A. A. Sobayo 1 and O. I. Kanyi 2 1 Department of Science and Technology, Bingham University, Karu, Nigeria. 2 Department of Aviation Medical, Federal Airport Authority Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Author BAA designed the study, performed the statistical analysis, wrote the protocol, and wrote the first draft of the manuscript. Author AAS managed the analyses of the study. Author OIK managed the literature searches. All authors read and approved the final manuscript. Article Information Editor(s): (1) Dr. Cynthia Aracely Alvizo Báez, Autonomous University of Nuevo Leon, Mexico. Reviewers: (1) Enyonam Tsolenyanu, University of Lome, Togo. (2) Alaaeldeen Balal Ahmed, Omdurman Islamic University, Sudan. Complete Peer review History: http://www.sdiarticle4.com/review-history/64335 Received 28 November 2020 Accepted 02 January 2021 Published 27 January 2021 ABSTRACT Aims: This study is aimed to determine the prevalence of Rotavirus among infants and young children that have gastroenteritis attending two Hospitals in Nasarawa State as a case study. Study Design: A Hospital-based Cross-sectional Study was used in this study, and demographic data was obtained using questionnaire. Place and Duration of Study: Department of Biological Science, Bingham University Karu, Nasarawa State, between May 2019 and July 2019. Methodology: 200 children of age 0-10 with gastroenteritis were included in the study. Stool samples were collected aseptically in a lick proof universal container and samples were screened for Rotavirus using faecal antigen Rotavirus EIA cassette kit according to the manufacture’s manual. The statistical analysis was done using the chi-square test (X 2 ) to determine the relationship between prevalence of Rotavirus and possible risk factors at 95% confident interval. Results: The prevalence rate of 8.5% was recorded. Children within the age group 0-3 had the highest frequency of 10.7% than other age groups. It was also observed that the infection was Original Research Article