Please cite this article in press as: Haddi A, et al. Polyunsaturated fatty acids affect intestinal anaphylactic response in BALB/c mice sensitized with -lactoglobulin. Rev Fr Allergol (2017), https://doi.org/10.1016/j.reval.2018.03.009 ARTICLE IN PRESS +Model REVAL-1210; No. of Pages 7 Disponible en ligne sur ScienceDirect www.sciencedirect.com Revue française d’allergologie xxx (2017) xxx–xxx Original article Polyunsaturated fatty acids affect intestinal anaphylactic response in BALB/c mice sensitized with -lactoglobulin Les acides gras polyinsaturés affectent la réponse anaphylactique intestinale chez la souris BALB/c sensibilisée à la -lactoglobuline A. Haddi ∗ , M. Guendouz , S. Ainad Tabet , N. Mehedi , O. Kheroua , D. Saidi Laboratory of Physiology of Nutrition and Food Safety, Department of Biology, Faculty of Natural and Life Sciences, University of Oran 1 Ahmed Ben Bella, BP 1524 ELM Naouer, Oran, Algeria Received 6 February 2018; accepted 22 March 2018 Abstract Objective. – Polyunsaturated fatty acids (PUFA) have immunomodulatory proprieties and their use in the prevention and treatment of allergy symptoms is proposed as a therapeutic option. The present study investigates the effect of n-3 and n-6 PUFA on intestinal anaphylactic response in mice allergic to -lactoglobulin (-lg), a major allergen in cow milk proteins. Material and methods. – Female BALB/c mice were fed by gavage for 15 days with either fish oil (FO) (n-3 PUFA) or corn oil (CO) (n-6 PUFA) at different concentrations (0.6%, 1%, 1.5% V/W) and were then sensitized with -lg. To study the local allergic manifestations in the intestine, electrophysiological parameters (short-current circuit, Isc, in A/cm 2 and tissue conductance, G, in mS/cm 2 ) were measured in jejunum segments in an Ussing chamber, while morphological changes were assessed by histological analysis. Results. – FO at 0.6% V/W significantly decreased Isc (A/cm 2 ) values (P < 0.05). All other doses of both oils proved ineffective. The three doses of FO (but not CO) significantly reduced tissue conductance (mS/cm 2 ) values (P < 0.05, P < 0.001). Histological analysis showed morphological improvement in all groups with increased villus height (P < 0.01, P < 0.001). Conclusion. – Low-dose PUFA supplementation, especially with n-3 PUFA, considerably reduced part of the intestinal damage resulting from sensitization with -lg in BALB/c mice. © 2018 Elsevier Masson SAS. All rights reserved. Keywords: n-3 PUFA; n-6 PUFA; -lactoglobulin; BALB/c; Ussing chamber Résumé Objectif. – Les acides gras polyinsaturés (AGPI) ont des propriétés immunomodulatrices, leur utilisation pour la prévention et le traitement des symptômes d’allergie est proposée comme une stratégie thérapeutique. Le présent travail étudie l’effet des AGPI n-3 et n-6 sur la réponse anaphylactique intestinale chez des souris rendues allergiques à la -lactoglobuline (-lg), un allergène majeur des protéines du lait de vache. Matériels et méthodes. – Des souris BALB/c femelles gavées pendant 15 jours avec de l’huile de poisson (FO) (AGPI n-3) ou de l’huile de maïs (CO) (AGPI n-6) à différentes doses : 0,6 %, 1 %, 1,5 % V/Pds sont ensuite sensibilisées avec la -lg. Pour étudier les manifestations allergiques locales au niveau intestinal, les paramètres électrophysiologiques ; le courant de court circuit Isc (A/cm 2 ) et la conductance tissulaire G (mS/cm 2 ) ont été mesurés en chambre de Ussing, une étude histologique a été aussi réalisée. ∗ Corresponding author. E-mail address: abira85@yahoo.fr (A. Haddi). https://doi.org/10.1016/j.reval.2018.03.009 1877-0320/© 2018 Elsevier Masson SAS. All rights reserved.