_____________________________________________________________________________________________________ *Corresponding author: E-mail: saritadas7@yahoo.com; European Journal of Medicinal Plants 22(2): 1-12, 2018; Article no.EJMP.38980 ISSN: 2231-0894, NLM ID: 101583475 Antiurobacterial Activity of Punica granatum L. Seed Extract Sarita Das 1* , Suchasmita Panigrahi 1 and Preetilata Panda 2 1 Microbiology Laboratory, Department of Botany, Berhampur University, Bhanja Bihar, Berhampur 760007, Odisha, India. 2 Department of Microbiology, MKCG Medical College, Berhampur, Odisha, India. Authors’ contributions This work was carried out in collaboration between all authors. Author SD designed the study, performed the statistical analysis, wrote the protocol and wrote the first draft of the manuscript. Authors SP and PP managed the analyses of the study. Authors SD and SP managed the literature searches. All authors read and approved the final manuscript. Article Information DOI: 10.9734/EJMP/2018/38980 Editor(s): (1) Daniela Rigano, Department of Chemistry of Natural Compounds, University Federico II of Naples, Italy. (2) Marcello Iriti, Professor, Plant Biology and Pathology, Department of Agricultural and Environmental Sciences, Milan State University, Italy. Reviewers: (1) Blanca Rosa Aguilar Uscanga, Universidad de Guadalajara, México. (2) Maria Demetriou, Democritus University of Thrace, Greece. Complete Peer review History: http://www.sciencedomain.org/review-history/23121 Received 3 rd November 2017 Accepted 27 th January 2018 Published 10 th February 2018 ABSTRACT The natural products derived from medicinal plants are proven to be the abundant source of biologically active compounds, which have been the basis of development of new pharmaceuticals. The present study was carried out to understand the antibacterial activity of methanolic extract of Punica granatum L. seed (MPG) against the clinically isolated urinary tract infections (UTI) causing bacteria i.e. Escherichia coli and Klebsiella pneumoniae using different methods i.e. disc diffusion, agar well diffusion, modified agar well diffusion. Colony forming units per ml (CFU/ml) were also determined for wild and MPG treated bacteria by spread plate and absorbance method. Phytochemical analysis of MPG and biochemical characterization of K. pneumoniae and E. coli was carried out using a standard protocol. MPG was a dark red colour sticky mass with a yield of 26.87% (w/w). Phytochemical analysis revealed the presence of steroids, tannins, coumarins, glycosides, flavonoids and phenolic compounds. The present investigation showed that MPG was effective against K. pneumoniae and E. coli. K. pneumoniae was a multi-drug resistant strain, found to be more sensitive to MPG in comparison to E. coli. The CFU/ml was found to be reduced in a Original Research Article