~ 173 ~ Journal of Entomology and Zoology Studies 2017; 5(3): 173-175 E-ISSN: 2320-7078 P-ISSN: 2349-6800 JEZS 2017; 5(3): 173-175 © 2017 JEZS Received: 24-03-2017 Accepted: 25-04-2017 Jyoti Sharma Govt. Degree College, Kathua, Jammu and Kashmir, India Shabir Ahmad Dar Govt. Degree College Baramulla, Jammu and Kashmir, India Seema Langer Department of Zoology, University of Jammu, Jammu and Kashmir, India AN Sayani College of Fisheries Junagadh Agricultural University, Veraval, Gujarat, India Correspondence Jyoti Sharma Govt. Degree College, Kathua, Jammu and Kashmir, India Seasonal variations in the spleen somatic index (SSI) of Garra gotyla gotyla Jyoti Sharma, Shabir Ahmad Dar, Seema Langer and AN Sayani Abstract In the present research, an attempt was made to study the seasonal variations in spleen somatic index (SSI) of Garra gotyla gotyla. Fish Garra gotyla gotyla selected for the present studies, were collected from the Jhajjar stream at Jhajjar Kotli one of the tributaries of river Tawi situated at an elevation of 400 meters above the mean sea level (msl) and is 34Km from Jammu on NH1A to Kashmir, the study was carried out from November,2009 to October, 2010. The lowest value of SSI was recorded during the summer season (0.150) and highest was observed during winter season (0.330). SSI exhibits an inverse relation with gonado somatic index (GSI) and total leucocyte count (TLC). The peak value of GSI was recorded during the summer season (9.31) whereas lowest value of 0.40 was noted during winter season. In similar manner TLC was highest during summer season (17.21×103/cmm) and lowest during winter season (14.29×103 /cmm). Keywords: Spleen somatic index, gonado somatic index, total leucocyte count, Garra gotyla gotyla. 1. Introduction The teleost spleen is a discrete organ containing in addition to erythrocyte sequestered from circulation, erythropoietic tissue involved in the synthesis of new erythrocytes [12] . Spleen is a major storage organ for blood cells and is known to contract in teleost fish during acute stress. Contraction of spleen results in release of red blood cells in blood and may account for increase in erythrocytes and lymphocytes [8] . So spleen appears to play an important role in haemopoiesis. Besides [2, 10] stated that production of antibodies showing immune reactivity in response to pathogens and foreign particles in blood stream is yet another important function of spleen. Spleen as a secondary lymphatic organ therefore is widely used as a simple measurable immunological variable with a potential role in immune responses against the entry of any foreign pathogen in fish [4, 3, 7] . Review of literature, however reveals that organ size is condition dependent including spleen [9, 3, 10] . A link between spleen size and fish condition has also been reported by workers like [9, 3] . From the state of art about spleen size it becomes apparently clear that though studies on spleen size and its role in haemopoiesis and immune reactivity is recognized but not much is available on its seasonal variations which can have many condition dependent influence on during life history in fishes [4] .Presently therefore an endeavor has been made to generate data on spleen size in percentage of body weight which is recognized as spleen somatic index (SSI) in fish Garra gotyla gotyla. 2. Material and Methods Adult specimens of Garra gotyla gotyla were collected with the help of cast net from Jhajjar stream a tributary of Chenab (J&K), India and were brought live to the laboratory and the study period was November, 2009 to October, 2010. Blood sample was collected with the help of disposable insulin syringes by making an incision through the heart of fish. Total leucocyte count (TLC) was counted with the help of improved Neubauer haemocytometer [5] . After taking blood, the fishes were dissected out and their gonads and spleen were taken out to study gonado somatic index (GSI) and spleen somatic index (SSI). Gonado somatic index which is the percentage of gonads in body weight was calculated using following formula [3] . GSI =weight of gonads (gm)/ weight of fish (gm) ×100 Spleen somatic index was determined by using the formula (SSI) = weight of spleen (gm)/weight of fish (gm) ×100