178 | Proc. of the 20 th FAVA CONGRESS & The 15 th KIVNAS PDHI, Bali Nov 1-3, 2018 Oral Presentation (PCS-13) The Profile and Morphology of Proboscis Monkey’s Blood Cells in South Kalimantan Irma Padeta 1 , Rini Widayanti 2 , Heri Budi Santoso 3 , Amalia Rezeki 4 , Teguh Budipitojo 1 * 1 Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl Fauna No. 2 Karangmalang, Yogyakarta, Indonesia, 55281 2 Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jl Fauna No. 2 Karangmalang, Yogyakarta, Indonesia, 55281 3 Program Study of Biology, Faculty of Mathematic and Science, Universitas Lambung Mangkurat, Jl. Brigjen H. Hasan Basri, Banjarmasin, South Kalimantan, Indonesia, 70124 4 Sahabat Bekantan Indonesia Foundation, Banjarmasin, South Kalimantan, Indonesia, 70123 *Corresponding author’s email : budipitojo@ugm.ac.id Keywords: blood cells, female, juvenile, Proboscis monkey, South Kalimantan. INTRODUCTION Twenty percent of primate species is found in Indonesia 1 . Proboscis monkey (Nasalis larvtaus) or bekantan is one of endemic species in Kalimantan 2,3 . This primate is belonging to Colobinae subfamily and distributed in Kalimantan (Indonesia), Sabah and Serawak (Malaysia), and Brunei. Proboscis monkey is belonging to endangered species based on International Union for the Conservation of Nature and Natural Resources (IUCN) assessment 4 . Indonesian Ministry of environment and forestry established Proboscis monkey is belonging to one of twenty five species which has been incrased in population. Blood cells have important role in body regulation such as maintaining body temperature, carriying oxygen, nutrition, hormone and metabolism product to the whole body 5 . Blood examination is important for body health evaluation 6,7 , especially in non-human primate where lived in zoo, animal captive or rehabilitiation center 6 . The aim of this study was to investigate the profile and morphology of female- juvenile proboscis monkey blood cells at rehabilitation center (Sahabat Bekantan Indonesia Foundation) in Banjarmasin, South Kalimantan. MATERIALS AND METHODS Blood sample was collected from four female-juvenile (3-5 years old) proboscis monkey from Sahabat Bekantan Indonesia Foundation, Banjarmasin, South Kalimantan. Sample was process for hematological examination and blood smear staining. Blood sample was examined in laboratory for eritrocytes, hemoglobin (Hb). leucocytes (neutrophils, eosinophils, basophils, monocytes and lymphocytes), platelet, pack cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC). The blood smear preparation were fixated by using methanol and then visualized by using Giemsa staining method. Blood cells morphology were observed and analyzed descriptively by using light microscope. RESULT AND DISCUSSION Proboscis monkey is endemic animal from Kalimantan and their existence was endangered. Zoo, animal captive or rehabilitation center establishment is concervation effort realization from government, public community, non-profit organization or educational institution for conserve the population. Sahabat Bekantan Indonesia (SBI) foundation is non-profit organization which collaborated with educational institution to supporting government program in proboscis monkey rehabilitation and concervation. Proboscis monkey’s health status monitoring in rehabilitation center were important. Blood cell profile is one of clinical parameter to monitoring and evaluating their healthy and effective treatment for unhealthy animals 6 . The female-juvenile proboscis monkey hematological examination result was showed in Table 1. The mean of eritrocytes value was 4,50 ± 0.07 10 6 /µL. The mean of erythrocytes indeces was Hb 11.6 ± 0.48 g/dL, PCV 35.9 ± 2.55 %, MCV 79.7 ± 5.22 fl, MCH 25,7 ± 0.90 pg, MCHC 32,2 ± 1.04 %. The mean of leucocytes value was 14.0 ± 3.12 10 3 /µL with differential basophils 1.3 ± 1.13 %, eosinophils 0,0 ± 0.05 %, neutrophils 36,7 ± 6.90 %, lymphocytes 58,9 ± 6.03 % and monocytes 3,8 ± 0.95 %. The mean of platelets 345.8 ± 153.64 10 3 /µL. There is no reference for female-juvenil proboscis monkey blood profile in rehabilitiation center. Macaques (Macaca mullata) and blood profile in captive animal has been reported for clinical health parameter 6 . Besides, Macaca fascicularis blood profile in their habitat were assessed for investigated physiological parameter for their utilization in research 8 . The result of this study could be hematology parameters for female- juvenil proboscis monkey in rehabilitiation center.