S347 Document heading In vitro antioxidant activities of jelly fish Chrysaora quinquecirrha venom from southeast coast of India K Suganthi, S Bragadeeswaran * , N Sri Kumaran, C Thenmozhi, S Thangaraj Centre of Advanced Study in Marine Biology,Annamalai University, Parangipettai - 608 502,Tamil Nadu, India Asian Pacific Journal of Tropical Biomedicine (2012)S347-S351 Asian Pacific Journal of Tropical Biomedicine journal homepage:www.elsevier.com/locate/apjtb *Corresponding author: K. Suganthi,Ph. D Research scholar, Centre of Advanced Study in Marine Biology, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India Tel:+ 91 9786970 955 E-mail: krishsuganthi@gmail.com 1. Introduction The jellyfish, Chrysaora quinquecirrha (C. quinquecirrha), a C nidarian of the class S cyphozoa, the order, Semaeostomeae and the family Pelagiidae is distributed widely in the Bay of Bengal, India. Toxicity is a common feature of Cnidarians and a large number of toxins have been identified in Cnidarian nematocysts. A variety of toxins are produced and used for prey capture and defense by the cnidarians, which include Anthozoa, Scyphozoa, Cubozoa and Hydrozoa. Toxins derived from some of the jelly fishes as a model for the development of new drug which also has promising applications in cardiovascular medicine and target medicine of nerve molecular biology [1] . Regarding jellyfish derived peptides, to the best of our knowledge there are only a few reports dealing with their antioxidant properties [2] . Antioxidants have an important role to protect the human body against damage by the free radicals. An antioxidant is a molecule capable of inhibiting other molecules oxidation. Oxidative-free radicals are byproducts of the normal reactions within our body. These reactions include the generation of calories, the degradation of lipids, the catecholamine response under stress, and the inflammatory processes. The interest in antioxidants has been increasing because of their high capacity in scavenging free radicals related to various diseases [3] . Various antioxidant compounds are identified in many natural sources including some protein compounds. Proteins of jellyfish and protein hydrolysates from different sources, such as milk protein, maize zein, egg-yolk, porcine proteins, yellow stripe trevally, yellow fin sole frame, mackerel, have been found to possess antioxidant activity [2] . Thousands of marine toxic organisms may be regarded as a precious treasure for man. A number of compounds from marine organisms possessing anti-bacterial, anti-coagulant, anti-diabetic, anti-inflammatory, antifungal, antimalarial, anti tuberculosis and anti-viral properties have recently ARTICLE INFO ABSTRACT Article history: Received 5 January 2012 Received in revised form 12 J anuary 2012 Accepted 23 February 2012 Available online 28 April 2012 Keywords: Chrysaora quinquecirrha Scavenging Antioxidant activities Superoxide radical Nitric oxide radical Hydroxyl radical Objective: To extract the venom from Chrysaora quinquecirrha (C. quinquecirrha) and tested for antioxidant potential of both crude and fractions. Methods: These venom proteins were purified through DEAE- cellulose column chromatography. The protein content from crude and fractionated samples (Frc 1 to Frc 3) showed 398, 180, 203 and 286 毺g/mL respectively. The molecular weight of purified protein were determined by SDS- PAGE and we found five distinct bands with molecular weight of 20, 35, 45, 70, 74 and 100 kDa in the crude 70 and 100 kDa in Frc-1, 21 and 68 kDa in Frc-2 and 14, 65 and 104 kDa in Frc-3. The in vitro antioxidant activity of crude and purified samples were evaluated in different assay systems Viz. radical scavenging activity by DPPH reaction, Superoxide radical scavenging activity in PMS/NADH-NBT system, hydroxyl radical by Fe 3+ -Ascorbate- EDTA, H2O2 system and Nitric oxide (NO) activity in sodium nitroprusside/Greiss Reagent system. Results: Fre-3 displayed the maximal antioxidant activity from all the assay system tested. Inhibitory activities in DPPH radicals 15%-92% Superoxide radicals 21%-96% hydroxyl radicals 30%-95% and Nitric oxide activity also showed significant activities. Conclusions: These results indicate that the jelly fish C. quinquecirrha venom have remarkable antioxidant activities. Further studies will fulfill for purification and structural elucidation. Contents lists available at ScienceDirect