ORIGINAL PAPER Hymenobacter persicinus sp. nov., a novel member of the family Hymenobacteraceae Leonid N. Ten . Na Yeong Jeon . Weilan Li . Myung Kyum Kim . Young-Je Cho . Hee-Young Jung Received: 10 December 2018 / Accepted: 18 January 2019 Ó Springer Nature Switzerland AG 2019 Abstract A bacterial strain, 1-3-3-3 T , was isolated from a soil sample collected in Jeollabuk-do province, South Korea. Cells were observed to be Gram-stain negative, short rod-shaped and colonies to be red-pink in colour. Analysis of 16S rRNA gene sequences identified this strain as a member of the genus Hymenobacter in the family Hymenobacteraceae, with high levels of 16S rRNA sequence similarity with Hymenobacter algoricola VUG-A23a T (98.0%), Hymenobacter knuensis 16F7C-2 (97.9%), Hy- menobacter fastidiosus VUG-A124 T (97.1%), Hy- menobacter elongatus VUG-A112 T (97.0%), Hymenobacter chitinivorans Txc1 T (97.0%) and Hy- menobacter aquaticus 16F3P T (96.7%). Growth of strain 1-3-3-3 T was observed at 10–30 °C, pH 6–8 and in the presence of 0–1.0% NaCl. The genomic G ? C content was determined to be 61.6 mol %. The predominant respiratory quinone of the isolate was found to be MK-7; the major fatty acids were identified as iso-C 15:0 (19.9%), summed feature 3 (C 16:1 x7c/C 16:1 x6c, 19.7%), summed feature 4 (iso- C 17:1 I/anteiso-C 17:1 B, 17.8%), C 16:1 x5c (12.5%) and anteiso-C 15:0 (11.2%), and the major polar lipid was found to be phosphatidylethanolamine. The pheno- typic and chemotaxonomic data support the affiliation of strain 1-3-3-3 T with the genus Hymenobacter. However, the DNA-DNA relatedness between the isolate and its closest phylogenetic neighbours was lower than 34%. The DNA–DNA hybridization result and the differentiating phenotypic properties clearly indicate that strain 1-3-3-3 T represents a novel species in the genus Hymenobacter, for which the name Hymenobacter persicinus sp. nov. is proposed. The type strain is 1-3-3-3 T (= KCTC 52742 T = JCM 32191 T ). Keywords Hymenobacter Á Hymenobacteraceae Á Polyphasic taxonomy Á Soil bacteria Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-019-01236-0) con- tains supplementary material, which is available to authorized users. L. N. Ten Á N. Y. Jeon Á W. Li Á H.-Y. Jung (&) School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea e-mail: heeyoung@knu.ac.kr M. K. Kim Department of Bio and Environmental Technology, Seoul Women’s University, Seoul 01797, Republic of Korea Y.-J. Cho School of Food Science and Biotechnology/Food and Bio- Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea H.-Y. Jung Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea 123 Antonie van Leeuwenhoek https://doi.org/10.1007/s10482-019-01236-0