275 Bulletin UASVM, Veterinary Medicine 65(1)/2008 pISSN 1843-5270; eISSN 1843-5378 PHYSICAL FACTORS ACTION ON NEISSERIA SPP. STRAINS ISOLATED FROM ANIMALS Răpuntean S., N. FiŃ, F. Chirilă, G. Nadă, Cuc Cosmina, Daniela Călina, Cristina Drig University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine 3-5 Mănătur Street, 400372, Cluj-Napoca, Romania; email: grapuntean@usamvcluj.ro Keywords: Neisseria; sensibility/resistance; physical factors. Abstract: Neisseria sp. strains were isolated from both diseased (rhinitis; conjunctivitis; otitis; dermatitis; mastitis) and healthy (cows; dogs; guinea pigs; rabbits) animals and identified based on the morphological; Gram; cultural and biochemical characters. 7 strains were identified as N. polysaccharea based on the biochemical examination on API NH system (BioMerieux). They were tested regarding sensibility/resistance to the following physical factors: 65 o C temperature; freezing - de- freezing; UV radiations; microwaves and ultrasounds. The researches are demonstrating that the tested strains are sensitive to temperature; UV radiations and microwaves; but highly resistant to freezing – de-freezing and ultrasounds. INTRODUCTION In nowadays classification neisseria is framed in Neisseriaceae family; genus Neisseria; comprising 29 species and subspecies. N. gonohorreae and N. meningitidis pathogen for humans are the most popular species; the other are considered as commensally on human and animal mucous membrane realizing a transitory and superficial relation without inducing specific antibody appearance (1; 3). The literature is mentioning frequent involvement of the Neisseria species other that N. gonohorreae and N. meningitidis in causing affections in organisms with various immune-suppression forms. In humans the genus is involved in endocarditis; meningitis; otitis; arthritis; toxemia; lungs affections; dental diseases; bite wounds complications; etc.; evolving as opportunistic infections in patients with immune-deficits (4; 6; 8). In animals kerato-conjunctivitis at sheep and goats (N. ovis); mastitis in cows; rhinitis and conjunctivitis in dogs and cats (N. canis; N. lactamica; N. flavescens; N. sicca; N. animalorum; N. zoodegmatis); diseases in laboratory animals (N. cuniculi; N. denitrificans; N. animalis; N. caviae); dental affections (N. dentiae) are frequently observed (8; 9). N. polysaccharea was recognized as taxon in 1983 (7). On the other hand the presence of commensally neisseria in culture media is creating differentiation difficulties for pathogen neisseria and other cocci (mostly micrococi; staphilococci); and for other Gram negative yellow pigmented bacillus (Flavobacterium; Alcaligenes) or species producing mucous colonies (Klebsiella; Acinetobacter). In the literature little data is referring to the sensibility of Neisseria sp. strains to various physical factors reason for that we tested strains isolated from animals to some of the mentioned factors. The results are applicable in annihilating neisseria from different materials; aliments; air and antigen preparing. MATERIAL AND METHODS Temperature. 5 test glass containing nutritive glucose liquid medium of 24 h culture were prepared (5 ml/test glass); of density 2 on McFarland scale. Cultural test glass were