_____________________________________________________________________________________________________ *Corresponding author: E-mail: kalu.erem@ebsu.edu.ng; Journal of Advances in Microbiology 20(4): 51-57, 2020; Article no.JAMB.56435 ISSN: 2456-7116 Antibacterial Activity of Honey on Staphylococcus aureus, Escherichia coli and Streptococcus pyogenes Isolated from Wounds V. U. Usanga 1 , M. O. Elom 1 , N. O. Umoh 2 , A. Nworie 1 , B. N. Ukwah 1 , M. E. Kalu 1* and S. O. Azi 1 1 Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Ebonyi State University, Abakaliki, Nigeria. 2 Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Cross River State, Nigeria. Authors’ contributions This work was carried out in collaboration among all authors. Author VUU designed the study, wrote the protocol and first draft of the manuscript. Authors MOE and BNU managed the analyses of the study. Authors AN, NOU and SOA managed the literature searches. Author MEK collected sample and managed the analyses too. All authors read and approved the final manuscript. Article Information DOI: 10.9734/JAMB/2020/v20i430236 Editor(s): (1) Dr. Pankaj Kumar, Dolphin (PG) Institute of Biomedical and Natural Sciences, India. Reviewers: (1) Jeferson Júnior da Silva, University of Campinas – UNICAMP, Brazil. (2) Phillip Minnaar, Agricultural Research Council of South Africa, South Africa. Complete Peer review History: http://www.sdiarticle4.com/review-history/56435 Received 17 February 2020 Accepted 23 April 2020 Published 23 May 2020 ABSTRACT Aim: To assess the antibacterial activity of crude honey obtained from Ishielu Local Government of Ebonyi State on pathogenic bacterial species (Staphylococcus aureus, Escherichia coli and Staphylococcus pyogenes) isolated from wounds. Study Design: An experimental study which involved a random selection of patients with wound Place and Duration of Study: Department of Medical Laboratory Science, Ebonyi State University, Abakaliki, Nigeria, between February 2019 and November 2019. Methodology: A total of 50 samples of wound swabs collected from different sites of open wounds were cultured on blood agar, chocolate agar and MacConkey agar. The crude honey was diluted to concentrations ranging from 20% to 100% and the antibacterial activity was carried out by well diffusion method with augmentin used as a control. Original Research Article