Iraqi Journal of Veterinary Sciences, Vol. 36, No. 3, 2022 (753-760) 753 Iraqi Journal of Veterinary Sciences www.vetmedmosul.com The beneficial effects of resveratrol supplementation on parasitemia, oxidative stress and serum biochemical parameters in Trypanosoma brucei infected dogs P. O. Akpa 1 , C.S. Ukwueze 2 , R.I. Odo 3 , C.J. Aronu 4 and B.M. Anene 1 1 Department of Veterinary Medicine, University of Nigeria, Nsukka, 2 Department of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, 3 Department of Veterinary Physiology and Pharmacology, University of Nigeria, 4 Department of Animal Health and Production, University of Nigeria, Nsukka, Nigeria Article information Abstract Article history: Received October 9, 2021 Accepted April 6, 2022 Available online June 10, 2022 This study investigated the effect of resveratrol supplementation on parasitemia, oxidative stress and serum biochemical changes in the Trypanosoma brucei infected dogs. The mean pre-patent period (MPP) of infection was 5.75±0.96 (5-7) days and 9.00 ± 0.82 (8-10) days between infected un-supplemented and supplemented respectively, which differed significantly (p<0.05). There was a significant increase (P<0.05) in the activities of malondialdehyde (MDA) post infection (pi) on the infected untreated group compared with the infected treated groups and the control. The mean serum alanine aminotransaminase (ALT) and aspartate aminotransferase (AST) were significantly (P>0.05) higher than the infected treated groups. The mean ALT of infected treated with both resveratrol and diminazene aceturate (DA) was significantly (P<0.05) lower than other infected treated groups. The mean creatinine and blood urea nitrogen (BUN) levels were significantly (P<0.05) higher in the infected untreated group. The creatinine level of the infected treated with both resveratrol and DA was significantly (P<0.05) lower than other infected treated groups, whereas the BUN did not differ significantly (P>0.05) within the infected treated groups when compared with the control. The mean albumin and total protein were significantly (P<0.05) higher in the infected untreated group, but the infected treated groups did not show any significant (P>0.05) difference. However, the total protein was significantly (P<0.05) higher in the infected treated with resveratrol than in other infected groups. It was concluded that pretreatment with resveratrol is beneficial in managing T. brucei infection, as it delays the onset of parasitemia and restores damages done to the liver and kidneys when combined with diminazene aceturate. Keywords: Resveratrol Supplementation Trypanosoma brucei Parasitemia Oxidative stress Correspondence: C.S. Ukwueze ukwueze.chigozie@mouau.edu.ng DOI: 10.33899/ijvs.2022.131843.2009, ©Authors, 2022, College of Veterinary Medicine, University of Mosul. This is an open access article under the CC BY 4.0 license ([available at]://creativecommons.org/licenses/by/4.0/). Introduction African animal trypanosomosis is a debilitating disease of humans and animals, often caused by T. brucei, and T. congolense in dogs (1,2). The disease is widespread and essential in Africa and sub-saharan African countries and affects a wide range of animals (3,4), leading to massive impact on economic losses, not only in dogs but in other animals as well, manifested as mortalities, reduced meat and milk yield in food animals and cost of treatment (5). Oxidative stress and inflammatory reactions have been suggested to play significant roles in the pathogenesis of trypanosomosis (6,7). Free radicals and many peroxides generated by trypanosomes and activated by mononuclear