non-stimulated and 49 stimulated saliva samples were tested for anti-HCV antibodies. Results were compared with HCV RNA detection in saliva and salivary glands. Statistical analysis was done. Results: Antibodies were detected in 26.1% of the saliva samples. In 23.5% of these, HCV RNA was detected. Of the 73.9% of saliva samples with undetectable antibodies, 36.8% were positive for HCV RNA. In 26.6% of the patients with salivary gland samples there were detectible antibodies in the saliva. Of these, 11.8% had HCV RNA in the salivary gland. From the 73.4% cases negative for salivary antibodies, 21.3% were positive for HCV RNA in the salivary gland. Conclusion: There was no correlation between anti-HCV antibodies in saliva and HCV RNA in saliva and salivary glands. Support: FAPEMIG. PE-367 - CYTOKERATINS AS PREDICTIVE BIOMARKERS FOR LIP CANCER IN PATIENTS WITH ACTINIC CHEILITIS. NATÁLIA GALVÃO GARCIA, DENISE TOSTES OLIVEIRA, MARIA APARECIDA CUSTÓDIO DOMINGUES, ELIANA MARIA MENICUCCI, CLÉVERSON TEIXEIRA SOARES. FACULDADE DE ODONTOLOGIA DE BAURU, UNIVERSIDADE DE SÃO PAULO (FOB/USP). This study evaluated the expression of CK10 and CK13 in 45 actinic cheilitis (AC) and 20 squamous cell carcinomas (SCC) of the lip samples and compared results with the cell proliferation index. Immunohistochemical analysis was per- formed using the antibodies anti-CK10, anti-CK13, and anti-Ki- 67. The association between the expression of CK10 and CK13 in AC and SCC was calculated using the Chi-square test. The results showed a loss of CK13 and absence of CK10 in dysplastic areas of AC. In SCC there was heterogeneous expression of CK13 and total absence of CK10. A statistically significant association was noted between the CK10 expression in SCC and AC with or without dysplasia (p < 0.001). These results suggest that CK13 and CK10 participate in the malignant transformation of AC, and that the absence or alteration of these biomarker expressions indicate dysplastic areas or SSC of the lip. Supported: CNPq-141641/2013-4. PE-368 - CYTOKINES AND DENDRITIC CELLS IN HU- MAN CHRONIC PERIODONTITIS. GIOVANNA RIBEIRO SOUTO, CELSO MARTINS QUEIROZ JUNIOR, MAURO HERINQUE NOGUEIRA GUIMARÃES DE ABREU, FERNANDO OLIVEIRA COSTA, RICARDO ALVES MESQUITA. FACULDADE DE ODONTOLOGIAeUFMG. This study evaluated cytokines and dendritic cells (DCs) on the human chronic periodontitis (CP). Study Design: Samples from 24 individuals with CP and 6 controls were obtained. Immunohistochemical examination was done, with cytokines IL-2, IL-10, IL-4, IL-6, IFN, TNF-a, and IL-17A measured using the cytometric bead array. DCs and inflammatory infiltrate densities, cytokines, classification of CP, and clinical parameters were correlated and compared. Results: IL-6 was positively correlated with the increase of CD1a+ DCs and probing depth (PD). IL-2 was negatively related to clinical attachment level (CAL), whereas positive correlations were observed between CD1a+ and factor XIIIA+ DCs with PD. IL-2, TNF-a, INF, IL-10, and IL-17A were increased when compared with con- trols. Conclusion: Although their levels were not increased in CP, IL-6 can inhibit DC maturation, resulting in increased levels of CD1a+ DCs. CNPq #309209/2010-2;472045/2011-3; FAPEMIG. PE-369 - DENTAL EXTRACTION IN HEAD AND NECK IRRADIATED PATIENTS UNDERGOING A CLINICAL- SURGICAL PROTOCOL TO PREVENT OSTEOR- ADIONECROSIS. MARIA CRISTINA MUNERATO, BRUNA JESINSKA SELBACH, MANOELA DOMINGUES MARTINS, MARCO ANTONIO TREVIZANI MARTINS. UFRGS/HCPA. Radiotherapy is the main treatment modality for patients with head and neck cancer. Osteoradionecrosis (ORN) is one of the most serious complications of radiotherapy, having as its main trigger dental extractions. This study evaluated the occurrence of ORN associated with dental extractions after radiotherapy, using a clinical-surgical protocol to prevent ORN. Methods: Twelve patients underwent 31 dental extractions. The protocol consisted of the use of antibiotics before and after dental extractions, use of chlorhexidine gluconate 0.12%, extraction with minimal trauma, osteoplasty, and first-intention suturing. Demographic data, tumor location, histology, radiation dose and time, number of teeth extracted, location, and repair evolution were assessed. Results: After 31 dental extraction procedures, 3 (9.67%) patients devel- oped ORN. There was no statistically significant correlation be- tween the development of ORN and the type of extraction and total dose of radiation received. Conclusion: The clinical-surgical protocol helped to reduce postoperative ORN in irradiated patients. PE-370 - DENTAL MINERALIZATION IN RICKETS STUDIED USING MICROCOMPUTED TOMOGRA- PHY. FÁBIO WILDSON GURGEL COSTA, THYCIANA RODRIGUES RIBEIRO, EDUARDO COSTA STUDART SOARES, JAMES CALDWELL WILLIAMS JR, CRISTIANE SÁ RORIZ FONTELES. UNIVERSIDADE FEDERAL DO CEARÁ. X-linked hypophosphatemic rickets (XLHR) is a rare con- dition characterized by alterations in vitamin D, phosphorus, and phosphate levels. Spontaneous abscesses are common oral find- ings. We evaluated dental mineralization in patients affected by XLHR using micro-computed tomography (mCT). Nineteen teeth from members of the same family (1 unaffected and 4 affected by XLHR) were collected and analyzed through mCT scan. Gender, age, tooth position, and tooth type (deciduous or permanent) were recorded for each patient. Projection images were reconstructed and analyzed using Anova. Affected individuals presented lower dentin mineralization values compared to unaffected patients. Gray values differed significantly between anterior and posterior teeth (p < 0.001), affected and unaffected (p < 0.001), and when position and disease status were considered (p < 0.001). Dentin mineralization was significantly reduced in patients affected by XLHR. Lower mineralization of the tooth matrix may contribute to the apparently poor ability of these teeth to resist infection. PE-371 - DIABETES MELLITUS: SYSTEMIC AND ORAL COMPLICATIONS IN 467 PATIENTS FROM CAPE- FOUSP. MARIA CAROLINA NUNES VILELA, CRISTINE AYAME IVANO, CÍNTIA DE PAULA MARTINS, KARIN SÁ FERNANDES, MARINA HELENA CURY GALLOTTINI, NATHALIE PEPE MEDEIROS DE REZENDE. FACUL- DADE DE ODONTOLOGIA DA UNIVERSIDADE DO ESTADO DO SÃO PAULO. Diabetes mellitus (DM) is a metabolic disorder characterized by impaired action or secretion of insulin or both, resulting in hyperglycemia with acute and chronic complications. We OOOO ABSTRACTS Volume 117, Number 2 Abstracts e203