Quantifying liquid-liquid phase separation property of chromatin under physiological conditions using Hi-MS and Hi-C Minglei Shi, 1, 8,* Kaiqiang You, 2,8 Chao Hou, 2 Taoyu Chen, 2 Zhengyu Liang, 4 Mingwei Liu, 5 Jifeng Wang, 6 Taotao Wei, 7 Jun Qin, 5 Yang Chen, 1,* Tingting Li, 2,9,* Michael Q. Zhang 1, 3,* 1, MOE Key Laboratory of Bioinformatics; Bioinformatics Division and Center for Synthetic & Systems Biology, BNRist; School of Medicine, Tsinghua University, Beijing 100084, China 2, Department of Biomedical Informatics, School of Basic Medical Sciences, Peking University Health Science Center, Beijing 100191, China 3, Department of Biological Sciences, Center for Systems Biology, The University of Texas, Richardson, TX 75080-3021, USA 4, Department of Cellular and Molecular Medicine, Institute of Genomic Medicine, University of California, San Diego, La Jolla, CA 92093, USA 5, State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing), Beijing Institute of Lifeomics, Beijing 102206, China 6, Laboratory of Proteomics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 7, National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China 8, These authors contributed equally 9, Lead Contact *Correspondence: shiml79@tsinghua.edu.cn (M.L.S.), yc@mail.tsinghua.edu.cn (Y.C.), litt@hsc.pku.edu.cn (T.T.L.), michael.zhang@utdallas.edu (M.Q.Z.) . CC-BY-NC-ND 4.0 International license available under a (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made The copyright holder for this preprint this version posted December 8, 2020. ; https://doi.org/10.1101/2020.12.07.415489 doi: bioRxiv preprint