Research Article Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum) Aatika Sikandar , 1 Mengyue Zhang, 1 Yuanyuan Wang, 2 Xiaofeng Zhu, 1 Xiaoyu Liu, 3 Haiyan Fan, 1 Yuanhu Xuan, 4 Lijie Chen, 1 and Yuxi Duan 1 1 Nematology Institute of Northern China, Shenyang Agricultural University, Shenyang 110866, Liaoning, China 2 College of Biosciences and Biotechnology, Shenyang Agricultural University, Shenyang 110866, Liaoning, China 3 College of Science, Shenyang Agricultural University, Shenyang 110866, Liaoning, China 4 College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, Liaoning, China Correspondence should be addressed to Yuxi Duan; duanyx6407@163.com Received 5 December 2019; Revised 26 February 2020; Accepted 6 March 2020; Published 30 March 2020 Academic Editor: Krishna K. Verma Copyright © 2020 Aatika Sikandar et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Antioxidants are the radical scavengers that inhibit peroxidation and other free-radical processes, which in return safeguard different organisms from various diseases attributed to radical reactions. Synthetic antioxidants inhibit free radicals, but they also have harmful side effects. However, mycochemicals of natural fungal origin are safe and best substitutes for harmful synthetic chemical antioxidants. e prime objectives of the study include appropriate qualitative and quantitative mycochemical screening, antioxidant potential, and chemical composition of Snef1216 (Penicillium chrysogenum). e study has used aluminium chloride colourimetric method, Folin–Ciocalteu reagent assay, and DPPH (1,1-diphenyl-1-picrylhydrazyl) for analysis of total flavonoid content and phenol content and antioxidant activity, respectively. However, the presence of biologically active compounds was screened through gas chromatography-mass spectrometry (GC-MS). Quantitative analysis demonstrated the existence of fla- vonoids, glycosides, flavones, saponins, phenols, and catecholic tannins excluding alkaloids, terpenoids, steroids, and gallic tannins. e outcomes exposed total flavonoid content and phenolic content in P. chrysogenum were 85.31 ± 1.23mg·QE/g and 135.77 ± 1.14mg·GAE/g, respectively. Snef1216 (P. chrysogenum) displayed the highest free-radical scavenging activity with 63.86% inhibition of DPPH. e analysis confirms that Snef1216 (P. chrysogenum) is an alternative source of natural antioxidants. e obtained data have provided the foundation for its use in agricultural, environmental, and pharmaceutical industries. 1. Introduction Free radicals are associated with numerous chronic and acute diseases in human beings such as asthma, athero- sclerosis cataracts, diabetes, liver injury, and neurodegen- erative disorder [1]. Antioxidants are useful constituents that are responsible for the inhibition of free radicals by abol- ishing their target site [2]. ey have the capability to capture free radicals such as hydroperoxide and peroxide which constrain oxidation and cause degenerative arrays [3]. Synthetic antioxidants inhibit free radicals and also are have harmful side effects [4]. However, natural resources of mycochemicals are safe and better substitutes for chemical antioxidants [5]. Fungi are an important source of natural antioxidants because of their natural ability to produce secondary metabolites [6]. ey contain antioxidants in the form of steroids, quinones, alkaloids, phenylpropanoids, tocopherol, phenols, tannins, lactones, terpenoids, tri- terpenes carotenoids, and flavonoids [7]. Essential mycochemicals contained in fungi such as phenolic contents have attained more interest due to their characteristics to inhibit disease by their antioxidant activity. Phenols and flavonoids are the major secondary metabolites of fungi [6]. Moreover, phenolic and flavonoids contents of Hindawi Journal of Analytical Methods in Chemistry Volume 2020, Article ID 3073906, 8 pages https://doi.org/10.1155/2020/3073906