Print ISSN 2537-0308 • Online ISSN 2537-0316 ADJ-for Girls, Vol. 6, No. 3, July (2019) — PP. 265: 273 The Official Publication of The Faculty of Dental Medicine For Girls, Al-Azhar University Cairo, Egypt. AL-AZHAR Dental Journal F o r G i r l s ABSTRACT Purpose: This study was designed to assess the chelating capacity of chitosan acetate and chitosan citrate on intraradicular dentin using environmental scanning electron microscope (ESEM) and quantity calcium content of intraradicular dentin using energy dispersive X-ray microanalyzer (EDX). Materials and Methods: sixty freshly extracted human single rooted lower premolars were instrumented with Revo-S fles till size AS 35.The samples were dispensed into 5 groups according to the fnal rinse used and control group (10 samples each); Group I: irrigated with 0.2% chitosan acetate, Group II: irrigated with 0.2% chitosan citrate, Group III: irrigated with 1% acetic acid, Group IV: irrigated with 1% citric acid, Group V: irrigated with 17% EDTA and control group which was divided into;(VIa) :5 samples irrigated with sterile saline and (VIb): 5 samples were neither prepared nor received any treatment. Samples were grooved longitudinally and the cleanliness of the root canals and smear layer were evaluated at three root levels using ESEM. The calcium content of intraradicular dentin of fve samples from each experimental group (I,II,III,IV,V) and the samples of control group (VIa and VIb) was measured using EDX. Results: At three root levels; there was a statistical difference among the tested groups and control group in the median scores of smear layer However, there was no statistical difference in the median scores of smear layer among the tested groups. Comparing the smear layer scores within each tested group, apical level has the highest median smear layer score compared to other two levels. Regarding calcium content, the highest mean calcium content was recorded in samples treated with saline followed by samples that did not receive any irrigation. However, no statistical difference among tested groups. Conclusion: chelating potential of 0.2% chitosan acetate and 0.2% chitosan citrate is comparable to that of 17% EDTA when used as fnal rinse without much altering the calcium content of intraradicular dentin compared to 17% EDTA. Codex : 34/1907 azhardentj@azhar.edu.eg http://adjg.journals.ekb.eg DOI: 10.21608/adjg.2019.6056.1048 KEYWORDS Chitosan acetate, chitosan citrate, calcium content, intraradicular dentin. * Paper extracted from master thesis titled “Evaluation of Chelating Potential of Chitosan Citrate and Chitosan Acetate on Intraradicular Dentin”. 1. Dentist at Ministry of Health. email: shaimaa514@yahoo.com 2. Professor of Endodontics, Endodontic Department, Faculty of Dental Medicine for Girls, Al –Azhar University. 3. Assistant Professor of Endodontics, Endodontic Department, Faculty of Dental Medicine for Girls, Al –Azhar University. Evaluation of Chelating Potential of Chitosan Citrate and Chitosan Acetate on Intraradicular Dentin Shaimaa A. Naem 1* , Mervat I. Fawzy 2 , Hagar A. Bastawy 3