Advances in Microbiology, 2021, 11, 63-74 https://www.scirp.org/journal/aim ISSN Online: 2165-3410 ISSN Print: 2165-3402 DOI: 10.4236/aim.2021.112005 Feb. 19, 2021 63 Advances in Microbiology Fecal Metabolomes in Response to Feed Supplemented with Fermented Parkia biglobosa and Sphenostylis stenocarpa in Obese Rats Olayinka Anthony Awoyinka 1* , Tolani Rachael 2 , Funmilola Comfort Oladele 1 , Margret Olutayo Alese 3 , Elijah Olalekan Odesanmi 4 , Daisi David Ajayi 5 , Gbenga Sunday Adeleye 6 , Bunmi Comfort Boyede 2 1 Department of Medical Biochemistry, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria 2 Department of Microbiology, Ekiti State University, Ado Ekiti, Nigeria 3 Department of Anatomy, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria 4 Department of Biochemistry, Ekiti State University, Ado Ekiti, Nigeria 5 Department of Chemical Pathology, Ekiti State University Teaching Hospital, Ado Ekiti, Nigeria 6 Department of Physiology, Ekiti State University, Ado Ekiti, Nigeria Abstract The ubiquitous consumption of junk foods has drastically contributed to the exponential rise in the incidence of obesity. Hence, the present study explores the therapeutic effect of selected indigenous wild bean Sphenostylis stenocar- pa (Otili) and condiment fermented Parkia biglobosa (Iru) on obese rats. The rats were fed with a high fat diet for four weeks and the gut microbiota was monitored every other day throughout the period of the experiment. Then, the fecal metabolome was analysed by Gas Chromatography Mass Spectroscopy (GC-MS). Although there was a decrease in the mean weight of rats treated with fermented iru compared with those given Otili, it was not statistically significantly (p ≤ 0.05). The organisms identified from the fecal samples of the fermented Iru groups are Proteus vulgaris, Bacillus cereus and Esherichia coli while those identified from the Otili group include Escherichia coli, and Citrobacter Freundii. However, further study revealed that Otili and Iru had a similar faecal metabolome. Medium chain fatty acids, such as Decanoic acid, Octanoic acid, ethyl tetradecanoate, Hexadecanoic acid, Methyl tetradeca- noate, 9-Hexadecanoic acid, Hexadecnoic acid, cis-10-Hepadecanoic acid, are the most common compounds found in this study. This suggests the fact that the associated gut microbiota from breakdown of respective food samples must have actively mediated in their roles of ameliorating the effect of obesity. How to cite this paper: Awoyinka, O.A., Rachael , T., Oladele, F.C., Alese, M.O., Ode- sanmi , E.O., Ajayi, D.D., Adeleye, G.S. and Boyede, B.C. (2021) Fecal Metabolomes in Response to Feed Supplemented with Fer- mented Parkia biglobosa and Sphenostylis stenocarpa in Obese Rats. Advances in Mi- crobiology, 11, 63-74. https://doi.org/10.4236/aim.2021.112005 Received: September 20, 2020 Accepted: February 16, 2021 Published: February 19, 2021 Copyright © 2021 by author(s) and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY 4.0). http://creativecommons.org/licenses/by/4.0/ Open Access