Vol.:(0123456789) 1 3
Cardiovascular Toxicology
https://doi.org/10.1007/s12012-019-09518-9
Combustion Particle‑Induced Changes in Calcium Homeostasis:
A Contributing Factor to Vascular Disease?
Jørn A. Holme
1
· Bendik C. Brinchmann
1
· Eric Le Ferrec
2
· Dominique Lagadic‑Gossmann
2
· Johan Øvrevik
1,3
© Springer Science+Business Media, LLC, part of Springer Nature 2019
Abstract
Air pollution is the leading environmental risk factor for disease and premature death in the world. This is mainly due to
exposure to urban air particle matter (PM), in particular, fne and ultrafne combustion-derived particles (CDP) from trafc-
related air pollution. PM and CDP, including particles from diesel exhaust (DEP), and cigarette smoke have been linked to
various cardiovascular diseases (CVDs) including atherosclerosis, but the underlying cellular mechanisms remain unclear.
Moreover, CDP typically consist of carbon cores with a complex mixture of organic chemicals such as polycyclic aromatic
hydrocarbons (PAHs) adhered. The relative contribution of the carbon core and adhered soluble components to cardiovas-
cular efects of CDP is still a matter of discussion. In the present review, we summarize evidence showing that CDP afects
intracellular calcium regulation, and argue that CDP-induced impairment of normal calcium control may be a critical cellular
event through which CDP exposure contributes to development or exacerbation of cardiovascular disease. Furthermore, we
highlight in vitro research suggesting that adhered organic chemicals such as PAHs may be key drivers of these responses.
CDP, extractable organic material from CDP (CDP-EOM), and PAHs may increase intracellular calcium levels by interacting
with calcium channels like transient receptor potential (TRP) channels, and receptors such as G protein-coupled receptors
(GPCR; e.g., beta-adrenergic receptors [βAR] and protease-activated receptor 2 [PAR-2]) and the aryl hydrocarbon receptor
(AhR). Clarifying a possible role of calcium signaling and mechanisms involved may increase our understanding of how air
pollution contributes to CVD.
Keywords Diesel exhaust particles · Polycyclic aromatic hydrocarbons · Endothelial dysfunction · Aryl hydrocarbon
receptor · Calcium signaling
Abbreviations
ADRs Adrenergic receptors
AhR Aryl hydrocarbon receptor
ARNT AhR nuclear translocator
B[a]P Benzo[a]pyrene
CVD Cardiovascular diseases
CDP Combustion-derived particles
CYP Cytochrome P450
[Ca
2+
]
i
Cytosolic concentration of calcium
DEP Diesel exhaust particles
DEP-EOM Extractable organic material of DEP
GPCRs G protein-coupled receptors
MMPs Matrix metalloproteinases
NF-κB Nuclear factor-κB
1-NP 1-nitropyrene
OC Organic chemicals
oxLDL Oxidized low-density lipoproteins
PM Particular matter
PAHs Polycyclic aromatic hydrocarbons
PAR-2 Protease-activated receptor 2
ROS Reactive oxygen species
ROCE Receptor-operated calcium entry
RTKs Receptor tyrosine kinases
Handling Editor: Travis Knuckles.
* Jørn A. Holme
jorn.holme@fhi.no
* Johan Øvrevik
Johan.Ovrevik@fhi.no
1
Department of Air Pollution and Noise, Division of Infection
Control, Environment and Health, Norwegian Institute
of Public Health, PO Box 4404, Nydalen, 0403 Oslo,
Norway
2
Univ Rennes, Inserm, EHESP, Irset (Institut de recherche
en santé environnement et travail) - UMR_S 1085,
35000 Rennes, France
3
Department of Biosciences, Faculty of Mathematics
and Natural Sciences, University of Oslo, Oslo, Norway