JJBS
Volume 3, Number 4, December 2010
ISSN 1995-6673
Pages 165 - 174
Jordan Journal of Biological Sciences
Nandrolone Decanoate Administration to Male Rats Induces
Oxidative Stress, Seminiferous Tubules Abnormalities, and
Sperm DNA Fragmentation
Lubna H. Tahtamouni
a,*
, Noor H. Mustafa
a
, Iman M. Hassan
a
, Iman M. Ahmad
b
,
Salem R. Yasin
a
, Maher Y. Abdalla
a,c
a
Deptartment of Biology and Biotechnology,
b
Deptartment of Radiography, The Hashemite University, P.O. Box 150459, Zarqa 13115,
Jordan;
c
Vaccine Branch, Center for Cancer Research, National Cancer Institute, National Naval Medical Center, Bldg. 8, Rm. 4155, 8901
Wisconsin Ave. Bethesda, MD, USA
Abstract اﻟﻤﻠﺨﺺ
The present study was conducted to evaluate the effects
of Nandrolone Decanoate (an anabolic steroid) on the
level of oxidative stress markers, sperm chromatin
integrity, seminiferous tubules structure, and
spermatogonia/Sertoli cell ratio in adult rat. Rats were
divided into three groups: control, low (3mg/Kg) and
high-dose (10mg/Kg) Nandrolone Decanoate-receiving
groups. Seminal fluid analysis was performed, and the
serum was used to evaluate testosterone level. Testicular
oxidative stress markers were measured and routine
histological preparation and androgen receptor
immunochemistry was used to evaluate the effects of
Nandrolone Decanoate injection on seminiferous tubules.
Injection of Nandrolone Decanoate caused an increase in
the production of thiobarbituric acid-reactive substances
in the testes of treated rats. The level of sperm DNA
fragmentation and the percentage of seminiferous tubules
showing maturation arrest were also increased in treated
animals. The absolute numbers of spermatogonia and
Sertoli cells in the rats receiving Nandrolone Decanoate
decreased significantly; however, the ratio of
spermatogonia/Sertoli cells did not. Administration of
anabolic steroids at supraphysiological doses leads to
multiple pathological changes in the reproductive system
of treated rats. Testosterone or its derivatives such as
Nandrolone Decanoate are being abused commonly.
Athletes, coaches, and physicians should be aware of
their harmful side effects.
ﺗﻬﺪف اﻟﺤﺎﻟﻴﺔ اﻟﺪراﺳﺔ إﻟﻰ ﺁﺛﺎر ﺗﻘﻴﻴﻢ ﺣﻘﻦNandrolone Decanoate
) اﻟﺒﻨﺎﺋﻴﺔ اﻟﺴﺘﻴﺮوﻳﺪات ﻣﻦ واﺣﺪ( ﻋﻠﻰ اﻻوآﺴﻴﺠﻴﻨﻲ، اﻟﺠﻬﺪ ﻣﺴﺘﻮى
وﺳﻼﻣﺔ اﻟﻮراﺛﻴﺔ اﻟﻤﺎدة ﻟ اﻟﻤﻨﻮﻳﺔ ﻠﺤﻴﻮاﻧﺎت و، اﻷﻧﺎﺑﻴﺐ وﻣﻜﻮﻧﺎت ﺷﻜﻞ
اﻟﻤﻨﻮﻳﺔ، اﻟﻤﺴﺎﻋﺪ ﻟﻠﺨﻼﻳﺎ اﻷم اﻟﻤﻨﻮﻳﺔ اﻟﺨﻼﻳﺎ وﻧﺴﺒﺔ ة) ﺳﺮﺗﻮﻟﻲ ﺧﻼﻳﺎ(
ﻓ ﺣﻘﻨﻬﺎ ﺗﻢ اﻟﺘﻲ اﻟﺠﺮذان ﻲ. ﻣﺠﻤﻮﻋﺎت ﺛﻼﺛﺔ إﻟﻰ اﻟﺠﺮذان ﺗﻘﺴﻴﻢ ﺗﻢ:
ﻣﺠﻤﻮﻋﺔ اﻟﻀﺒﻂ ﻣﻦ ﻣﺘﺪﻧﻲ ﺑﺘﺮآﻴﺰ ﺣﻘﻨﺖ ﻣﺠﻤﻮﻋﺔ وDecanoate
Nandrolone ) 3 ﻣﺞ/ آﻐﻢ( ﻋﺎﻟﻲ ﺑﺘﺮآﻴﺰ ﺣﻘﻨﺖ ﻣﺠﻤﻮﻋﺔ و
) 10 ﻣﺞ/ آﻐﻢ.( أن إﻟﻰ اﻟﺪراﺳﺔ ﺗﻮﺻﻠﺖ اﻹ ﻟﻤﺮآﺐ اﻟﻤﻔﺮط ﺳﺘﺨﺪام
Nandrolone Decanoate إﻟﻰ أدى إﻓﺴﺎد اﻟﺤﻤ اﻟﻨﻮوي ﺾ ﻟﻠ ﺤﻴﻮاﻧﺎت
اﻟﻤﻨﻮﻳﺔ و إﻳﻘﺎ إﻟﻰ ف ﻣﺨﺘﻠﻔﺔ ﻣﺮاﺣﻞ ﻓﻲ اﻟﻤﻨﻮﻳﺔ اﻟﺤﻴﻮاﻧﺎت ﺗﻜﻮﻳﻦ ﻋﻤﻠﻴﺔ.
ﻓﻲ ﺗﻐﻴﻴﺮ أي ﻳﺤﺪث وﻟﻢ ﺳﺮﺗﻮﻟﻲ، ﺧﻼﻳﺎ إﻟﻰ اﻷم اﻟﻤﻨﻮﻳﺔ اﻟﺨﻼﻳﺎ ﻧﺴﺒﺔ
اﻟﻤﻄﻠﻖ اﻟﻌﺪد وﻟﻜﻦ آﺒﻴﺮ ﺑﺸﻜﻞ ﻗﻞ اﻟﺨﻼﻳﺎ ﻟﻬﺬﻩ. ذﻟﻚ، إﻟﻰ وﺑﺎﻹﺿﺎﻓﺔ
اﻟﺒ اﻟﺴﺘﻴﺮوﻳﺪات ﺣﻘﻦ ﻨ ﺗﺴﺒﺐ ﺎﺋﻴﺔ ﻓﻲ زﻳﺎدة إﻧﺘﺎجAcid
Thiobarbituric دون أي ﺣﺪوث ﺗﻐﻴﻴﺮ ﻓﻲ ﻣﺴﺘﻮىGSH ﺧﺼﻴﺔ ﻓﻲ
اﻟﺠﺮاذﻳﻦ ب ﺣﻘﻨﺖ اﻟﺘﻲNandrolone Decanoate . اﺳﺘﻌﻤﺎل إﺳﺎءة إن
اﻟﺒﻨﺎﺋﻴﺔ اﻟﺴﺘﻴﺮوﻳﺪات ﺗﺼﺒﺢ ﺑﺪأت أﺳﺎﺳﻴﺔ ﻣﺸﻜﻠﺔ اﻟﻌﺎﻣﺔ اﻟﺼﺤﺔ ﻣﺠﺎل ﻓﻲ
اﻟﺘﻌﻠﻴﻤﻴﺔ اﻟﺒﺮاﻣﺞ ﺗﻨﻔﻴﺬ ﺿﺮورة ﻳﻌﻨﻲ ﻣﺎ وهﻮ ﻟ ﺘﻮﻋﻴ ﺔ اﻟﻤﺮاهﻘ وﺗﺤﺬﻳﺮ ﻴﻦ
واﻟﻤﺮﺷﺪﻳﻦ ﺳﻮاء ﺣﺪ ﻋﻠﻰ اﻟﺠﺎﻧﺒﻴﺔ اﻵﺛﺎر ﻋﻦ و اﻟﺴﻠﺒﻴﺔ اﻟﻌﻘﺎرات ﻟﻬﺬﻩ
ﻣﺴﺘﺨﺪﻣﻴﻬﺎ ﺻﺤﺔ ﻋﻠﻰ.
© 2010 Jordan Journal of Biological Sciences. All rights reserved
Keywords: Germinal Epithelium, TBARS, Glutathione, Anabolic Steroids, Sertoli Cells, Androgen Receptor.
1. Introduction
*
Anabolic-androgenic steroids (AAS) are synthetic
compounds which are based on the structure of
testosterone, and are used to treat various conditions such
*
Corresponding author. lubnatahtamuni@hu.edu.jo.
as reproductive system dysfunction, breast cancer and
anemia (Thiblin and Petersson, 2005). Three basic
modifications are made to the structure of testosterone to
enhance deliverability and potency and slow down rate of
degradation. The first of these modifications (class I)
involves esterification of testosterone at the 17-β-hydroxy
location (Hall and Hall, 2005). This modification, which is
made to injectable AAS, slows down degradation but
enhances androgenic properties (Hall and Hall, 2005).