Int.J.Curr.Microbiol.App.Sci (2017) 6(6): 2924-2931 2924 Original Research Article https://doi.org/10.20546/ijcmas.2017.606.347 Evaluation of Fracture Healing Property of Ethanolic Extract of Cissus quadrangularis Using 3-Point Bending Model in Rats Reena R. Giri 1 , Kiran R. Giri 2* and Kamlesh Palandurkar 3 1 Department of Pharmacology, RIMS, Rajnandgaon C.G., India 2 Department of Pharmacology, 3 Department of Biochemistry, BHU, Varanasi, India *Corresponding author ABSTRACT Introduction Bone is a very active tissue and that any injury to it will induce a series of biological and biochemical changes (Udupa et al., 1964). The chemical analysis of Cissus quadrangularis revealed a high concentration of vitamin C, carotene A, a substance like anabolic steroid and calcium. It has been known for long time for its specific action on fracture healing (Udupa et al., 1964; 1960), hence selected for observation of its actual effect on the process of fracture healing judged by radiological examination in experimental animals (rats). Materials and Methods The present study was conducted after the approval of research protocol by the Institutional Animal Ethical Committee, Jawaharlal Nehru Medical College, Sawangi (Meghe), Wardha. Cissus quadrangularis purchased from local herbarium Nagpur”, identified and authenticated with the standard sample preserved as Cissus quadrangularis, by Botany Department, R. T. M. University Nagpur. The fleshy stems of CQ washed and cut into small pieces, air dried. Then 4 kg of chopped pieces of Cissus quadrangularis was soaked in 6 L of Ethyl Alcohol 99.9 % for 48 h, and the extract was filtered and allowed to air dry. International Journal of Current Microbiology and Applied Sciences ISSN: 2319-7706 Volume 6 Number 6 (2017) pp. 2924-2931 Journal homepage: http://www.ijcmas.com A study was undertaken to evaluate the fracture healing effect of ethanolic extracts of Cissus quadrangularis on experimentally fractured tibia of rats radiologically by using 3-point bending model. Closed transverse fracture of the mid – diaphysis of left tibia were created in rats after Phenobarbitone & Ketamine anaesthesia, by 3- point bending method. In the skiagram there was an evidence of union in Cissus quadrangularis treated group with extensive bony deposition compared to that of control [Normal saline] group. In our study, the findings assessed on the basis of radiological studies showed that ethanolic extract of Cissus quadrangularis had a definite fracture healing effect. The extracts can also be utilized as a new source of bone healer in the treatment of fracture. Keywords Closed transverse Fracture, Skiagram. Accepted: 26 May 2017 Available Online: 10 June 2017 Article Info