International Journal of Health Sciences and Research Vol.12; Issue: 5; May 2022 Website: www.ijhsr.org Original Research Article ISSN: 2249-9571 International Journal of Health Sciences and Research (www.ijhsr.org) 200 Vol.12; Issue: 5; May 2022 Standardization of Siddha Formulation Soothagavaayu Kudineer Chooranam M. Sangeetha 1 , B. Bharathi 2 , V. Velpandian 3 , M. D. Saravanadevi 4 1 PG Scholar, Postgraduate Department of Gunapadam, Government Siddha Medical College, Arumbakkam, Chennai-106, Tamilnadu, India 2 PG Scholar, Postgraduate Department of Gunapadam, Government Siddha Medical College, Arumbakkam, Chennai-106, Tamilnadu, India 3 Professor, Postgraduate Department of Gunapadam, Government Siddha Medical College, Arumbakkam, Chennai-106, Tamilnadu, India. 4 Professor and Head of the Department, Postgraduate Department of Gunapadam, Government Siddha Medical College, Arumbakkam, Chennai-106, Tamilnadu, India Corresponding Author: M. Sangeetha DOI: https://doi.org/10.52403/ijhsr.20220522 ABSTRACT Soothagavaayu kudineer chooranam is one of the polyherbal formulation mentioned in siddha system of medicine. The main objective of the study was to evaluate the quality of formulation Soothagavaayu kudineer chooranam by conducting Physicochemical, Biochemical and Phytochemical evaluation through advanced analytical techniques. The organoleptic nature of the drug provides the purity and quality of the formulation. The results obtained from physicochemical evaluation shows that the total ash value of Soothagavaayu kudineer was 2±0.1%, in which the acid insoluble ash was 3.1±1.94%,loss on drying at 105ºC of the formulation Soothagavaayu kudineer chooranam was noted to be7.43±0.97 in which water soluble extract value and alcohol soluble extract value was 33.9±1.34 and 0.085% respectively. Biochemical analysis of Soothagavaayu kudineer chooranam exhibit the appearance of carbonates and nitrates. High performance Thin Layer Chromatography analysis of Soothagavaayu kudineer chooranam reveals the appearance of two prominent peaks corresponds to presence of two versatile phytocomponents present within it. Rf value of the peaks ranges from 0.07 to 0.20. Phytochemical analysis of Soothagavaayu kudineer chooranam reveals the emergence of phytocomponents like Alkaloids, Flavonoids, Steroids, Triterpenoids, Coumarin, Phenol, Tannin, Protein, Saponins, Sugar, Betacyanin. In conclusion, the polyherbal formulation of Soothagavaayu kudineer chooranam possess significant phytocomponents and have beneficial effects towards treating various disorders. Keywords: Standardization, Decoction, Physicochemical, Soothagavaayu kudineer chooranam, Polyherbal. 1. INTRODUCTION In favour of the use of medicinal plants, they are the only resource available in nature which have comparatively few side effects. Synthetic drug in general has potent pharmacodynamic effects, but many also have strong and possibly dangerous harmful side effects. Nowadays Siddha treatment towards common people were well established and seek more attention about knowing herbal remedies and treatment procedures. Siddha system of medicine was indicated for the management of various ailments. There are 32internal medicines available in Siddha literature. In that decoction is considered to be one of the most effective dosage forms in Siddha system of medicine. Its life span was only 3hours and have to be used in fresh state due to loss of its therapeutic action (1) .