Physicochemical Characterisation, Drug Polymer Dissolution
and in Vitro Evaluation of Phenacetin and Phenylbutazone Solid
Dispersions with Polyethylene Glycol 8000
SHERAZ KHAN,
1
HANNAH BATCHELOR,
2
PETER HANSON,
1
YVONNE PERRIE,
1
AFZAL R. MOHAMMED
1
1
Aston Pharmacy School, Aston University, Birmingham B4 7ET, UK
2
Heart of England NHS Foundation Trust, Birmingham B9 5SS, UK
Received 11 March 2011; revised 12 April 2011; accepted 20 April 2011
Published online 10 May 2011 in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/jps.22613
ABSTRACT: Poor water solubility leads to low dissolution rate and consequently, it can limit
bioavailability. Solid dispersions, where the drug is dispersed into an inert, hydrophilic polymer
matrix can enhance drug dissolution. Solid dispersions were prepared using phenacetin and
phenylbutazone as model drugs with polyethylene glycol (PEG) 8000 (carrier), by melt fusion
method. Phenacetin and phenylbutazone displayed an increase in the dissolution rate when
formulated as solid dispersions as compared with their physical mixture and drug alone coun-
terparts. Characterisation of the solid dispersions was performed using differential scanning
calorimetry (DSC), Fourier transform infrared spectroscopy (FTIR) and scanning electron mi-
croscopy (SEM). DSC studies revealed that drugs were present in the amorphous form within
the solid dispersions. FTIR spectra for the solid dispersions of drugs suggested that there
was a lack of interaction between PEG 8000 and the drug. However, the physical mixture of
phenacetin with PEG 8000 indicated the formation of hydrogen bond between phenacetin and
the carrier. Permeability of phenacetin and phenylbutazone was higher for solid dispersions
as compared with that of drug alone across Caco-2 cell monolayers. Permeability studies have
shown that both phenacetin and phenylbutazone, and their solid dispersions can be categorised
as well-absorbed compounds. © 2011 Wiley-Liss, Inc. and the American Pharmacists Association
J Pharm Sci 100:4281–4294, 2011
Keywords: solid dispersions; PEG 8000; Phenacetin; Phenylbutazone; FTIR; DSC; Dissolu-
tion studies; Permeability; amorphous form
INTRODUCTION
Poor solubility of drugs in water and gastrointestinal
fluids leads to low dissolution rates and consequently,
it can limit bioavailability. Bioavailability of a drug is
dependent on its solubility and permeability.
1
Tech-
niques that have commonly been used to improve
dissolution and bioavailability of poorly water-soluble
drugs include salt formation,
2
micronisation,
3
the use
of surfactants,
4
particulate delivery systems
5
and the
formulation of solid dispersions.
6
Solid dispersions
are defined as the dispersions of one or more active
pharmaceutical ingredients in an inert hydrophilic
Correspondence to: Afzal R. Mohammed (Telephone: +44-121-
2044183; Fax: +44-121-2044000; E-mail: a.u.r.mohammed@aston
.ac.uk)
Journal of Pharmaceutical Sciences, Vol. 100, 4281–4294 (2011)
© 2011 Wiley-Liss, Inc. and the American Pharmacists Association
carrier in solid state. It is a widely used technique
to enhance drug dissolution and in turn, it promotes
bioavailability of insoluble drugs. Solid dispersions
in water-soluble carriers have attracted considerable
interest as a means of improving dissolution and
bioavailability of a range of hydrophobic drugs.
7
The
enhanced drug dissolution can be ascribed to multiple
factors, including conversion of crystalline drug into
amorphous form, reduction in particle size and an im-
proved wettability due to inert hydrophilic carrier.
8
The fabrication of solid dispersions is associated
with the complexity of the release mechanism and
the multitude of factors that can affect it, includ-
ing the properties of drug,
9
particle size,
10
proper-
ties of the polymer forming the matrix,
11
molecular
weight,
12
physical state
13
and possible drug–polymer
interaction.
14
Theories proposed for the drug release
mechanisms from solid dispersions are dependent on
the dissolution of both drug and polymer. Two widely
JOURNAL OF PHARMACEUTICAL SCIENCES, VOL. 100, NO. 10, OCTOBER 2011 4281