332 Int. J. Medical Engineering and Informatics, Vol. 9, No. 4, 2017
Copyright © 2017 Inderscience Enterprises Ltd.
Cell cocultures on coated scaffolds applied to liver
models
Odin Ramírez-Fernández*, Rafael Godínez
and Esmeralda Zuñiga-Aguilar
Departamento de Ingeniería Eléctrica,
Universidad Autónoma Metropolitana,
Unidad Iztapalapa, Apdo,
Postal 55-534, Iztapalapa, México
Email: odinramirezfernandez@gmail.com
Email: rgodinezfer@gamil.com
Email: esza08@gmail.com
*Corresponding author
Luis E. Gómez-Quiroz and
María C. Gutiérrez-Ruiz
Departamento de Ciencias de la Salud,
Universidad Autónoma Metropolitana,
Unidad Iztapalapa, Apdo,
Postal 55-534, Iztapalapa, México
Email: legq@xanum.uam.mx
Email: mcgr@xanum.uam.mx
Juan Morales and Roberto Olayo
Departamento de Física,
Universidad Autónoma Metropolitana,
Unidad Iztapalapa, Apdo,
Postal 55-534, Iztapalapa, México
Email: jmor@xanum.uam.mx
Email: oagr@xaum.uam.mx
Abstract: Volumetric cell cultures are widely used as an experimental model
to mimic the characteristics and responses of cell in tissues in vivo.
Hepatocytes, particularly, are highly specialised cells, their geometrical
conformation, and their coculture with no parenquimal cells and vascularised
tissue, are essential to their physiology and proper function. Polymeric
scaffolds for cell growth have been modified through plasma polymerisation to
improve their biological properties. This work presents a Poly (L-lactic acid)
(PLLA) coated scaffold with polypyrrole plasma doped with iodine (Ppy-I).
The scaffold where seeded with two different cell lines, hepatocytes (HepG2)
with stellate cells (Ito), and hepatocytes (HepG2) with endothelial cells
(HUVEC), the data were evaluated against control hepatocytes (HepG2)
culture. The morphology of the cells was evaluated by microscopy; to
corroborate the cell proliferation on the material, images of the samples with