ORIGINAL ARTICLE Effect of Myo-inositol on sperm quality and biochemical factors in cryopreserved semen of patients with Asthenospermia Maryam Azizi 1 | Ebrahim Cheraghi 2 | Malek Soleimani Mehranjani 1 1 Department of Biology, Faculty of Sciences, Arak University, Arak, Iran 2 Department of Biology, Faculty of Sciences, University of Qom, Qom, Iran Correspondence Malek Soleimani Mehranjani, Department of Biology, Faculty of Science, Arak University, Sardasht, P.O. Box: 381-5688138 Arak, Iran. Email: m-soleimani@araku.ac.ir Funding information Arak University Abstract In this study, the influence of myoinositol (MYO) as an antioxidant on the inhibition of the negative impacts of cryopreservation on sperm quality in men with Asthenos- permia was investigated. In this prospective study, each semen sample from 25 cases was separated into three groups: Fresh, Control (with freezing medium), Myoinositol (2 mg/ml). According to the World Health Organization criteria (WHO) (2010), total motility, progressive sperm motility, viability, normal morphology, and DNA integrity were assessed. In addition, the hypo-osmotic swelling (HOS) test and mitochondrial membrane potential (MMP) were used. Total antioxidant capacity (TAC), malondialde- hyde (MDA), and antioxidant enzyme activity were determined by the ELISA method. In contrast to the fresh samples, lipid peroxidation, DNA integrity damage, DNA frag- mentation, HOST, and MMP had significant enhancement in the control samples. Sperm quality was significantly decreased (p < 0.05). Mean percentage viability, normal morphology, total motility, progressive motility, and DNA integrity were significantly enhanced in the MYO group in comparison to the control group (p < 0.05). The MDA and TAC levels and DNA damage in the MYO group were significantly lower compared to the control group (p < 0.05). The findings confirm that sperm quality in patients with Asthenospermia is improved by the administration of 2 mg/ml of myoinositol together with the freezing medium after sperm cryopreservation. KEYWORDS Asthenospermia, cryopreservation, myoinositol, sperm quality 1 | INTRODUCTION A proper method of controlling and protecting male fertility in domestic animals and humans today is sperm cryopreservation (Sharma, 2011). Testicular failure or ejaculatory dysfunction can be caused by cytotoxic treatments, including chemotherapy, radiother- apy, and surgical therapies (Rousset-Jablonski et al., 2016). Under such conditions, sperm freezing is an appropriate method to protect fertility. The frozen–thawed sperm is used for assisted reproductive technology (ART) and has a backup sperm source (Dohle, 2010). Despite the general use of cryopreservation in ART, sperm survival is still limited and does not meet ideal expectations (Kefer et al., 2009). In addition, cryopreservation disrupts mitochondrial function and damages DNA integrity in reproductive cells (Johnston et al., 2012; Kopeika et al., 2015). Due to the mismatch of the production of ROS with the content of antioxidants, cryopreservation is a reason for oxidative stress (OS) (Agarwal et al., 2005). In addition, the lack of cytoplasmic protec- tive mechanisms makes spermatozoa susceptible to oxidative stress. This manifests as oxidation of cellular components and damage to cel- lular structure (including the plasma membrane, DNA, and acrosome), ultimately leading to reduced fertility (O'connell et al., 2002). Received: 7 January 2022 Revised: 29 April 2022 Accepted: 26 June 2022 DOI: 10.1111/and.14528 Andrologia. 2022;e14528. wileyonlinelibrary.com/journal/and © 2022 Wiley-VCH GmbH. 1 of 8 https://doi.org/10.1111/and.14528