S- 1 - Supporting Information for : Understanding the fate of environmental chemicals inside living organisms: NMR based 13 C isotopic suppression selects only the molecule of interest within 13 C enriched organisms Daniel Lane, Yalda Liaghati Mobarhan, Ronald Soong, Paris Ning, Wolfgang Bermel, Maryam Tabatabaei Anaraki, Bing Wu, Hermann Heumann, § Marcel Gundy, § Holger Boenisch, § Tae-Yong Jeong, Vera Kovacevic, Myrna J. Simpson, André J. Simpson,* ,† Department of Physical and Environmental Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, ON, Canada, M1C 1A4 Department of Chemistry, University of Toronto, 80 St. George St., Toronto, ON, Canada, M5S 3H6 Bruker BioSpin GmbH. Silberstreifen 4 , 76287 Rheinstetten, Germany § Silantes GmbH, 80339 München, Germany Corresponding Author *(andre.simpson@utoronto.ca) Table of Contents Experimental……………………………………………………………………………… Pages S2 Factors impacting the limit of detection and quantification of 12 C metabolites...……Pages S2-4 1D X Filtering NMR Pulse Sequence……………………………………………………Pages S5-11 Figure S2……………………………………………………………………………………Page S12