Thomas et al. Volume 2 (2), 2014, Page-173-178 173 © International Journal of Pharma Research and Health Sciences. All rights reserved e-ISSN: 2348-6465 Original Article Isolation and characterisation of mosquitolarvicidal compound from Gliricidia sepium Jacq. Jiji Thomas 1,*, Shonima Govindan M 1 , Muraleedhara Kurup G 2 1 School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India. 2 Dept. of Biochemistry, Kariavattom, Kerala University, Trivandrum, Kerala, India. ARTICLE INFO ABSTRACT _____________________________________________________________________________________ 1. Corresponding author * Jiji Thomas, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India. 1. INTRODUCTION The Mosquitoes, commonly called “flying syringes”, as they are sanguivorous vectors, cause more sufferings to the humans than any other organism. It is not time to forget the recent rampage of Chikungunya and Dengue fever all over the state, pushing the people into death or International Journal of Pharma Research and Health Sciences Available online at www.pharmahealthsciences.net Received: 01 Apr 2014 Accepted: 20 Apr 2014 Objective: Isolation and identification of larvicidal phytochemical from the plant Gliricidia sepium, which is commonly used for smouldering to repel mosquitoes. Methods: Larvicidal activity of petroleum ether, hexane, acetone, methanol and water extracts of Gliricidia sepium leaves were assayed for toxicity against 4 th instar larvae of Culex quinquefasciatus. The larval mortality was observed after 24 h exposure. The crude petroleum ether extract was further purified by column chromatography and eluates were tested for larvicidal activity. Selected one was identified by spectral analysis. Result: In the present study, bioassay- guided fractionation of G.sepium leaf extract led to the separation and identification of 8,11,14- eicosatrienoic acid as a potential new mosquitolarvicidal compound with LC 50 value 0.011 mg/ml and LC 90 as 0.060 mg/ml against 4 th instar larvae of Culex quinquefasciatus. GC-MS, FTIR, 1 H NMR and 13 C NMR spectral analysis confirmed the identification of active compound. Conclusion: This work could grab success in extricating ourselves by emanating a safe eco friendly solution from the plant Gliricidia sepium. As the source plant is ubiquitous in Kerala zone and the method of extraction is not any way cumbrous it may be easily manufactured and launched into the market for the effective application. Key words: Gliricidia sepium, Culex quinquefasciatus, eicosatrienoic acid, Larvicidal activity