_____________________________________________________________________________________________________ *Corresponding author: E-mail: kunal.gokhle@dlhhcop.org; Journal of Pharmaceutical Research International 33(58B): 284-302, 2021; Article no.JPRI.79162 ISSN: 2456-9119 (Past name: British Journal of Pharmaceutical Research, Past ISSN: 2231-2919, NLM ID: 101631759) Formulation Development of Novel Curcumin Analogue Loaded Non-aqueous Gel and Curcumin Analogue Loaded Nanoparticle (CA-NP) Gel for Topical Use and In-vitro Antioxidant Study Kunal M. Gokhale a* and Dhara S. Pandey a a Dr. L. H. Hiranandani College of Pharmacy, Ulhasnagar, MH, India. Authors’ contributions This work was carried out in collaboration between both authors. Both authors read and approved the final manuscript. Article Information DOI: 10.9734/JPRI/2021/v33i58B34204 Open Peer Review History: This journal follows the Advanced Open Peer Review policy. Identity of the Reviewers, Editor(s) and additional Reviewers, peer review comments, different versions of the manuscript, comments of the editors, etc are available here: https://www.sdiarticle5.com/review-history/79162 Received 10 October 2021 Accepted 14 December 2021 Published 15 December 2021 ABSTRACT In this paper we have reported development of suitable dosage form for novel curcumin analogue synthesized in our laboratory. The work involves preformulation studies of synthesized curcumin analogue followed by preparation and optimization of non-aqueous gel and curcumin loaded (CA- NP) nanoparticle gel. The formulated gels were observed visually for clarity, homogeneity, and phase separation. They were tested for their appearance and presence of any aggregates. Curcumin analogue loaded PLGA nanoparticles were prepared by using the nanoprecipitation - solvent evaporation method and further optimized. In-vitro antioxidant activity of formulation was then evaluated using DPPH radical scavenging activity. The gel exhibited good antioxidant activity with IC 50 value of 5.39 μg/ml. Keywords: Curcumin analogue; Non-aqeuous gel; PLGA nanoparticle; antioxidant activity. 1. INTRODUCTION Curcumin is very versatile in showing biological activities ranging from treatment for skin wounds to angiogenic effect. It is also well accepted fact that curcuminoids have many limitations in terms of metabolism, low bioavailability, inadequate absorption, systemic elimination. Hence, we Original Research Article