Research Article Evaluation of Antioxidant, Cytotoxic, Anti-Inflammatory, Antiarthritic, Thrombolytic, and Anthelmintic Activity of Methanol Extract of Lepidagathis hyalina Nees Root Shafiqul Islam, 1 Fowzul Islam Fahad, 1 Arifa Sultana, 2 Syed Al Jawad Sayem, 1 Shawon Baran Roy, 1 Mohammad Nazmul Islam , 1 Arpita Roy , 3 and Mohammed Abu Sayeed 1 1 Department of Pharmacy, International Islamic University Chittagong, Chittagong-4318, Bangladesh 2 Department of Pharmacy, Faculty of Pharmacy, University of Dhaka, Dhaka-1000, Bangladesh 3 Department of Biotechnology, School of Engineering & Technology, Sharda University, Greater Noida, India Correspondence should be addressed to Mohammad Nazmul Islam; nazmul@iiuc.ac.bd, Arpita Roy; arbt2014@gmail.com, and Mohammed Abu Sayeed; pmasayeed@yahoo.com Received 31 December 2021; Revised 17 January 2022; Accepted 20 January 2022; Published 15 February 2022 Academic Editor: Ruchika Garg Copyright © 2022 Shafiqul Islam et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Lepidagathis hyalina Nees is an ethnomedicinally potential Asian herb, locally used to treat cardiovascular diseases and coughs. e study was intended to evaluate qualitative and quantitative investigation to ensure numerous pharmacological properties of methanol extracts of L. hyalina Ness root (MELHR). MELHR manifested strong radical scavenging activity in the reducing power and DPPH (1, 1-diphenyl-2-picrylhydrazyl) assays, and phenol and flavonoid in the quantitative assays. In the study of the thrombolytic assay, MELHR showed moderate explicit percentage of clot lysis (29.39 ± 1.40%)withmoderate(135.35 µg/mL) toxic properties. e in vitro anti-inflammatory activity was evaluated by the inhibition of hypotonicity-induced RBC hemolysis, whereas the plant extract exhibited a significant (pp 0.005) dose-dependent inhibition and the highest inhibition was found 55.01 ± 3.22% at 1000 µg/mL concentration. Moreover, the MELHR also showed significant (p < 0.005) dose-dependent po- tentiality on protein denaturation which is considered as antiarthritic activity, and the peak inhibition was found significant (71.97 ± 2.71%) at 1000 µg/mL concentration. MELHR also exhibited the dose-dependent and statistically significant anthelmintic potential on aquarium worm (Tubifextubifex). So, the present investigation suggests that L. hyalina couldbethebestchoiceforthe management of cardiovascular, inflammation, arthritis, and anthelmintic diseases. Further investigation should be necessary to determine behind the mechanism of bioactivity and therapeutic potential of this plant. 1. Introduction Free radicals are major concern for biological evolution and also have certain beneficial effects on some species [1]. An imbalance between the accumulation and production of ROS in tissues causes oxidative stress [2, 3]. Oxidative stress triggers inflammation, which in chronic conditions results in atherosclerosis formation, thrombosis, plaque rupture, myocardial injury, and failure like serious cardiac diseases [4, 5]. Different epidemiological studies express that some inflammatory mediators not only facilitate the proliferation of malignant cells in the microenvironment of the tumor, but also induce metastasis and angiogenesis and redefine the responses of hormones, chemotherapeutic agents, and overall adaptive immunity [6]. Some bioactive compounds such as capsaicin, catechins, lycopenes, cucurbitacin B, isoflavones, phenethyl isothiocyanate, benzyl isothiocyanate, and piper- longumine have been proved to exert effective pharmaco- logical activities to treat cancer [7]. Certain phytochemicals like thymol, berberine, curcumin, lycopene, epigallocatechin, resveratrol, vanillin, and sulforaphane may also reduce the possibility of the onset of several types of cancer [8–11]. Hindawi Evidence-Based Complementary and Alternative Medicine Volume 2022, Article ID 2515260, 10 pages https://doi.org/10.1155/2022/2515260