S158 Abstracts / Toxicology Letters 211S (2012) S43–S216 Results and conclusions: Mice lethality was recorded from the dose of 90 g PLTX/kg, alone or in combination with OA. Gross pathology revealed mainly swelling of gastro-intestinal tract. Ani- mals treated with PLTX showed dose-dependent decrease in liver weight, that was not influenced by the presence of OA and did not recovered after 14 days of withdrawal. Moreover, some alteration was also recorded in aspartate and alanine transaminases (AST and ALT) and glutammate dehydrogenase (GLDH). On the whole, the possibility of a co-contamination should be carefully considered when deciding regulatory limits for PLTX in seafood. doi:10.1016/j.toxlet.2012.03.572 P24-07 Morphological and histological changes in kidneys induced by BDE209 and Cd Marijana Curcic 1 , Vesna Jacevic 2 , Sasa Jankovic 3 , Slavica Vucinic 2 , Ksenija Durgo 4 , Nevenka Kopjar 5 , Biljana Antonijevic 1 1 Faculty of Pharmacy, Belgrade University, Serbia, 2 Military Medical Academy, Serbia, 3 Inst. og Meat Hygiene and Technology, Serbia, 4 Faculty of Food Technology and Biotechno, Croatia, 5 Inst. og Medical Research, Croatia Purpose of this study was to examine whether the kidneys are the targets for the effects of mixture of decabromodiphenyl ether (BDE 209) and cadmium (Cd). Wistar rats (200–240 g) were exposed orally to BDE 209 (1000 mg/kg/bw), Cd (7.5 mg/kg/bw) or their combinations, by gav- age, for 28 days. Controls exposed to DMSO or saline were also included in the experiment. The following end points were exam- ined: kidney weight, morphological and histological changes in kidneys. Study was approved by Ethical Committee of Military Medical Academy (No. 9687/11). Kidney weight increased only in the group administered by BDE209 (approximately 10%). Serious morphological changes in kidneys were not observed in samples from rats exposed to the mixture, however patohistological analysis of kidney cortex indi- cated edema and hyperemia, with clearly limited bleeding. In all glomerules Bowman’s capsule was thickened. Most of capillaries were dilated and surrounded with cell infiltrate. Epithelial cells of proximal and distal tubules were very swollen. Eosinophilic, very grainy appearance of the cytoplasm could be seen in all cells degen- erate. Swollen nuclei of these cells are located in the central or apical part of cells. By quantification of histological observations using the scale from 0 to 5 the following results were obtained: saline 0.1, DMSO 0.16, BDE209 1.16, Cd 2.13 and mixture 2.87. Obtained results have indicated that all treated groups induced qualitatively similar histological effects, but the mixture was more potent than Cd and BDE209 per se. (Ministry of Science, III 46009.) doi:10.1016/j.toxlet.2012.03.573 P24-08 Effects of BDE209 and Cd mixture on liver in sub-acutely exposed rats Marijana Curcic 1 , Sanja Stankovic 1 , Sasa Jankovic 2 , Slavica Vucinic 3 , Vesna Jacevic 3 , Ksenija Durgo 4 , Biljana Antonijevic 1 1 Faculty of Pharmacy, Belgrade University, Serbia, 2 Inst. og Meat Hygiene and Technology, Serbia, 3 Military Medical Academy, Serbia, 4 Faculty of Food Technol Bitecchnol, Croatia The aim of this study was to examine hepatotoxic effect of the mixture of decabromodiphenyl ether (BDE 209) and heavy metal cadmium (Cd) in rats. Wistar rats (200–240 g) were exposed orally to BDE 209 (1000 mg/kg/bw), Cd (7.5 mg/kg/bw) or their combinations, by gav- age, for 28 days. Control groups exposed to DMSO or saline were also included in the experiment. Hepatotoxicity was assessed based on: liver weight, morphology, histology and liver enzymes activi- ties (aspartate aminotransferase – AST, alanine aminotransferase – ALT and -glutamyltransferase – -GT). Study was approved by Ethical Committee of Military Medical Academy (no. 9687/11). Liver weight increased in all treated groups according to the fol- lowing order: Cd group (40%) > Cd + BDE209 group (29%) > BDE209 group (15%). Morphological changes in liver were not observed while histological analysis indicated degenerative changes in hep- atocytes, hyperemia, vascular bleedings and disruption of vascular membranes. There were no changes in AST values. ALP was signifi- cantly lower in Cd group, while in Cd + BDE209 and BDE209 groups decrease in ALP activity was not so intensive like in Cd group. Activ- ity of -GT was higher in the mixture group than in groups where Cd or BDE 209 was administered alone. Obtained results are a lit- tle controversial since degenerative changes were not followed by increase in AST and ALT activities. More intensive toxic response to the mixture was seen in relation to effects on histology and -GT. (Ministry of Science, Project 46009.) doi:10.1016/j.toxlet.2012.03.574 P24-09 Biological interactions in mixtures of chemical substances Zdenek Fiala 1 , Vladimir Krajak 2 , Jan Kremlacek 3 , Peter Bednarcik 4 , Lenka Borska 3 , Ondrej Fiala 5 , Adolf Vyskocil 6 , Francois Lemay 6 , Tomas Borsky 5 1 Charles University, Faculty of Medicine, Czech Republic, 2 Regional Hygienic Institute, Czech Republic, 3 CHarles University, Faculty of Med, Czech Republic, 4 University of Defence, Fac. of Mil, Czech Republic, 5 Medtec-VOP Ltd., Czech Republic, 6 University of Montreal, Canada Purpose: Chemical substances in occupational atmosphere are often present in a form of mixtures. Estimation of final biologi- cal effect of mixtures is often very complicated. Uncertainty of the determination of biological interactions in mixtures represents a significant problem in the process of health risk assessment. Methods: The data of 3653 cases of working exposures were evaluated (chemical, biological and physical factors). In this group we found 720 cases of mixtures of chemical substances in occupa- tional atmosphere (two and more substances). Possible biological interactions in these mixtures were estimated by using of the database “miXie” (Czech modification of the original database “miXie”, IRSST Montreal). The database works on the principle of “classes of similar effects”. All effects on given organs or systems are considered similar, irrespective of the degree of specificity of this effect. Results: In the mixtures we identified 215 substances. Very often there were toluene, xylenes, acetone, gasoline and formaldehyde. In 687 mixtures (from a total number of 720), at least one combina- tion of two or more substances with potential aditivity was found (the same class of similar effects). Most often was found upper res- piratory tract irritation (C2), eye disorder (C1), lower respiratory tract disorder (C3) and central nervous system disorder (C19). A total number of 63 mixtures showed potential supra-aditivity and 3 mixtures potential infra-aditivity.